Effects of vitamin D and retinoic acid on human glioblastoma cell lines

L. Magrassi, G. Butti, S. Pezzotta, L. Infuso, G. Milanesi

Research output: Contribution to journalArticlepeer-review

Abstract

The biological significance of vitamin D receptors expressed by glioblastoma and other glial tumours is still unclear. In an effort to clarify this issue we studied the effects of increasing concentrations of 25-dihydroxyvitamin D3 and its metabolite 1 α, 25-dihydroxyvitamin D3 on two human glioblastoma cell lines. Both substances were capable of inducing a significant (> 50%) reduction in growth of the two glioblastoma cell lines at dosages over 5 μM. When the HU 70 cell line was treated by increasing dilutions of 25-dihydroxyvitamin D3 combined with 1 μM all trans-retinoic acid, significant inhibition was apparent even after addition of 25-dihydroxyvitamin D3 in the nanomolar range. Reduction of growth index was mainly due to induced cell death. Our results provide in vitro evidence that vitamin D metabolites alone or in combination with retinoids may be potentially useful agents in the differentiation therapy of human malignant gliomas.

Original languageEnglish
Pages (from-to)184-190
Number of pages7
JournalActa Neurochirurgica
Volume133
Issue number3-4
DOIs
Publication statusPublished - Sep 1995

Keywords

  • cell death
  • Glioblastoma
  • retinoic acid
  • vitamin D

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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