Effects of molecular weight and surface functionalization on surface composition and cell adhesion to Hyaluronan coated titanium

M. Morra, C. Cassinelli, A. Carpi, R. Giardino, M. Fini

Research output: Contribution to journalArticle


This paper describes the effect of surface functionalization on surface composition and cell adhesion to titanium samples by high and low molecular weight Hyaluronan (HA). HA was covalently linked to aminated Ti surfaces obtained by two different surface functionalization techniques, that is polyethyleneimine (PEI) adsorption and deposition from allylamine plasma. The two approaches yield very different surface densities of available amino groups, affecting this way the number and frequency of surface-HA bonds and the configurational freedom of the latter. Results of cell adhesion test are dependent on the surface functionalization approach adopted, low molecular weight HA coupled to PEI functionalized Ti does not yield the same degree of resistance to cell adhesion found on other samples. These results indicate that the details of the surface functionalization step are crucial for surface engineering of implant devices by biological molecules.

Original languageEnglish
Pages (from-to)365-369
Number of pages5
JournalBiomedicine and Pharmacotherapy
Issue number8
Publication statusPublished - Sep 2006



  • Hyaluronan (HA)
  • Polyethyleneimine (PEI)
  • Surface analysis
  • Surface modification
  • Titanium

ASJC Scopus subject areas

  • Pharmacology

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