Efficacy of antibacterial-loaded coating in an in vivo model of acutely highly contaminated implant

Gianluca Giavaresi, Enzo Meani, Maria Sartori, Andrea Ferrari, Davide Bellini, Anna C. Sacchetta, Joachim Meraner, Andrea Sambri, Caterina Vocale, Vittorio Sambri, Milena Fini, Carlo L. Romanò

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: The purpose of this study was to test the ability of DAC®, a fast resorbable, antibacterial-loaded hydrogel coating, to prevent acute bacterial colonization in an in vivo model of an intra-operatively highly contaminated implant. Methods: A histocompatibility study was performed in 10 adult New Zealand rabbits. Then, methicillin-resistant Staph. aureus were inoculated in the femur of 30 adult New Zealand rabbits at the time of intra-medullary nailing; vancomycin-loaded DAC® coated nails were compared to controls regarding local and systemic infection development. Results: Histocompatibility study showed no detrimental effect of DAC® hydrogel on bone tissue after 12 weeks from implant. After seven days from implant, none of the rabbits receiving vancomycin-loaded DAC® nail showed positive blood cultures, compared to all the controls; vancomycin-loaded DAC® coating was associated with local bacterial load reduction ranging from 72 to 99 %, compared to controls. Conclusions: Vancomycin-loaded DAC® coating is able to significantly reduce bacterial colonization in an animal model of an intra-operatively highly contaminated implant, without local or general side effect.

Original languageEnglish
Pages (from-to)1505-1512
Number of pages8
JournalInternational Orthopaedics
Volume38
Issue number7
DOIs
Publication statusPublished - 2014

Keywords

  • Coating
  • DAC
  • Hydrogel
  • Implant
  • Infection
  • Nail
  • Prosthesis

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Medicine(all)

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