Abstract
We evaluated the in vitro effect on clonogenic potential (CFU-GM) and apoptosis in myelodysplastic syndromes (MDS) progenitors of an anti-oxidant (N-acetylcysteine, NAC) and/or a differentiating (all-trans retinoic acid, ATRA) agent. NAC significantly reduced apoptosis, both NAC and ATRA induced an increase in CFU-GM, but NAC seemed to be particularly effective in the high risk (HR) MDS. NAC+ATRA conferred a significant advantage in terms of CFU-GM with respect to NAC and ATRA alone. Tumor Necrosis Factor-α (TNF-α) levels decreased after incubation with NAC in the MDS samples. This study shows that ineffective hemopoiesis in MDS could benefit from both NAC and ATRA, suggesting that anti-oxidant treatment may play a role in guaranteeing MDS cell survival, predisposing them towards differentiation. Copyright (C) 2000 Elsevier Science Ltd.
Original language | English |
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Pages (from-to) | 129-137 |
Number of pages | 9 |
Journal | Leukemia Research |
Volume | 24 |
Issue number | 2 |
DOIs | |
Publication status | Published - Feb 2000 |
Keywords
- All-trans retinoic acid
- Apoptosis
- Clonogenic activity
- Myelodysplastic syndromes
- N-acetylcysteine
ASJC Scopus subject areas
- Cancer Research
- Hematology
- Oncology