Abstract
We evaluated the in vitro effect on clonogenic potential (CFU-GM) and apoptosis in myelodysplastic syndromes (MDS) progenitors of an anti-oxidant (N-acetylcysteine, NAC) and/or a differentiating (all-trans retinoic acid, ATRA) agent. NAC significantly reduced apoptosis, both NAC and ATRA induced an increase in CFU-GM, but NAC seemed to be particularly effective in the high risk (HR) MDS. NAC+ATRA conferred a significant advantage in terms of CFU-GM with respect to NAC and ATRA alone. Tumor Necrosis Factor-α (TNF-α) levels decreased after incubation with NAC in the MDS samples. This study shows that ineffective hemopoiesis in MDS could benefit from both NAC and ATRA, suggesting that anti-oxidant treatment may play a role in guaranteeing MDS cell survival, predisposing them towards differentiation. Copyright (C) 2000 Elsevier Science Ltd.
| Original language | English |
|---|---|
| Pages (from-to) | 129-137 |
| Number of pages | 9 |
| Journal | Leukemia Research |
| Volume | 24 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2000 |
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Keywords
- All-trans retinoic acid
- Apoptosis
- Clonogenic activity
- Myelodysplastic syndromes
- N-acetylcysteine
ASJC Scopus subject areas
- Cancer Research
- Hematology
- Oncology
Cite this
Efficacy of N-acetylcysteine and all-trans retinoic acid in restoring in vitro effective hemopoiesis in myelodysplastic syndromes. / Cortelezzi, Agostino; Cattaneo, Chiara; Sarina, Barbara; Cristiani, Silvia; Pomati, Mauro; Silvestris, Ilaria; Motta, Marina; Ibatici, Adalberto; Gornati, Gianluca; Della Volpe, Aldo; Teresa Maiolo, Anna.
In: Leukemia Research, Vol. 24, No. 2, 02.2000, p. 129-137.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Efficacy of N-acetylcysteine and all-trans retinoic acid in restoring in vitro effective hemopoiesis in myelodysplastic syndromes
AU - Cortelezzi, Agostino
AU - Cattaneo, Chiara
AU - Sarina, Barbara
AU - Cristiani, Silvia
AU - Pomati, Mauro
AU - Silvestris, Ilaria
AU - Motta, Marina
AU - Ibatici, Adalberto
AU - Gornati, Gianluca
AU - Della Volpe, Aldo
AU - Teresa Maiolo, Anna
PY - 2000/2
Y1 - 2000/2
N2 - We evaluated the in vitro effect on clonogenic potential (CFU-GM) and apoptosis in myelodysplastic syndromes (MDS) progenitors of an anti-oxidant (N-acetylcysteine, NAC) and/or a differentiating (all-trans retinoic acid, ATRA) agent. NAC significantly reduced apoptosis, both NAC and ATRA induced an increase in CFU-GM, but NAC seemed to be particularly effective in the high risk (HR) MDS. NAC+ATRA conferred a significant advantage in terms of CFU-GM with respect to NAC and ATRA alone. Tumor Necrosis Factor-α (TNF-α) levels decreased after incubation with NAC in the MDS samples. This study shows that ineffective hemopoiesis in MDS could benefit from both NAC and ATRA, suggesting that anti-oxidant treatment may play a role in guaranteeing MDS cell survival, predisposing them towards differentiation. Copyright (C) 2000 Elsevier Science Ltd.
AB - We evaluated the in vitro effect on clonogenic potential (CFU-GM) and apoptosis in myelodysplastic syndromes (MDS) progenitors of an anti-oxidant (N-acetylcysteine, NAC) and/or a differentiating (all-trans retinoic acid, ATRA) agent. NAC significantly reduced apoptosis, both NAC and ATRA induced an increase in CFU-GM, but NAC seemed to be particularly effective in the high risk (HR) MDS. NAC+ATRA conferred a significant advantage in terms of CFU-GM with respect to NAC and ATRA alone. Tumor Necrosis Factor-α (TNF-α) levels decreased after incubation with NAC in the MDS samples. This study shows that ineffective hemopoiesis in MDS could benefit from both NAC and ATRA, suggesting that anti-oxidant treatment may play a role in guaranteeing MDS cell survival, predisposing them towards differentiation. Copyright (C) 2000 Elsevier Science Ltd.
KW - All-trans retinoic acid
KW - Apoptosis
KW - Clonogenic activity
KW - Myelodysplastic syndromes
KW - N-acetylcysteine
UR - http://www.scopus.com/inward/record.url?scp=0034142546&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034142546&partnerID=8YFLogxK
U2 - 10.1016/S0145-2126(99)00165-4
DO - 10.1016/S0145-2126(99)00165-4
M3 - Article
C2 - 10654448
AN - SCOPUS:0034142546
VL - 24
SP - 129
EP - 137
JO - Leukemia Research
JF - Leukemia Research
SN - 0145-2126
IS - 2
ER -