Efficacy of N-acetylcysteine and all-trans retinoic acid in restoring in vitro effective hemopoiesis in myelodysplastic syndromes

Agostino Cortelezzi, Chiara Cattaneo, Barbara Sarina, Silvia Cristiani, Mauro Pomati, Ilaria Silvestris, Marina Motta, Adalberto Ibatici, Gianluca Gornati, Aldo Della Volpe, Anna Teresa Maiolo

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated the in vitro effect on clonogenic potential (CFU-GM) and apoptosis in myelodysplastic syndromes (MDS) progenitors of an anti-oxidant (N-acetylcysteine, NAC) and/or a differentiating (all-trans retinoic acid, ATRA) agent. NAC significantly reduced apoptosis, both NAC and ATRA induced an increase in CFU-GM, but NAC seemed to be particularly effective in the high risk (HR) MDS. NAC+ATRA conferred a significant advantage in terms of CFU-GM with respect to NAC and ATRA alone. Tumor Necrosis Factor-α (TNF-α) levels decreased after incubation with NAC in the MDS samples. This study shows that ineffective hemopoiesis in MDS could benefit from both NAC and ATRA, suggesting that anti-oxidant treatment may play a role in guaranteeing MDS cell survival, predisposing them towards differentiation. Copyright (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)129-137
Number of pages9
JournalLeukemia Research
Volume24
Issue number2
DOIs
Publication statusPublished - Feb 2000

Keywords

  • All-trans retinoic acid
  • Apoptosis
  • Clonogenic activity
  • Myelodysplastic syndromes
  • N-acetylcysteine

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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