Efficacy of novel plasmid DNA encoding vaccinia antigens in improving current smallpox vaccination strategy

Miguel Otero, Sandra A. Calarota, Anlan Dai, Anne S. De Groot, Jean D. Boyer, David B. Weiner

Research output: Contribution to journalArticle

Abstract

We tested a DNA vaccine strategy in order to improve the efficacy and safety of the current live smallpox vaccine involving priming with DNA vaccines and boosting with live vaccinia virus (VacV). We generated DNA plasmids encoding the A4L, A27L and H5R VacV genes. A considerable increase in antigen-specific IFN-γ responses, high proliferative and humoral antigen-specific responses were detected in experimental primed Balb/C mice compared to controls after VacV boost. The VacV-DNA plasmids elicited IFN-γ production in HLA-A2.1 transgenic mice in response to predicted HLA-A2.1 restricted peptide epitopes, providing valuable data for further vaccine development.

Original languageEnglish
Pages (from-to)4461-4470
Number of pages10
JournalVaccine
Volume24
Issue number21
DOIs
Publication statusPublished - May 22 2006

Keywords

  • Cellular and humoral immune responses
  • DNA vaccines
  • Smallpox

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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    Otero, M., Calarota, S. A., Dai, A., De Groot, A. S., Boyer, J. D., & Weiner, D. B. (2006). Efficacy of novel plasmid DNA encoding vaccinia antigens in improving current smallpox vaccination strategy. Vaccine, 24(21), 4461-4470. https://doi.org/10.1016/j.vaccine.2005.08.010