Efficacy of tachyplesin III, colistin, and imipenem against a multiresistant Pseudomonas aeruginosa strain

Oscar Cirioni, Roberto Ghiselli, Carmela Silvestri, Wojciech Kamysz, Fiorenza Orlando, Federico Mocchegiani, Fabio Di Matteo, Alessandra Riva, Jerzy Łukasiak, Giorgio Scalise, Vittorio Saba, Andrea Giacometti

Research output: Contribution to journalArticlepeer-review

Abstract

An experimental study has been performed to compare the in vitro activity and the in vivo efficacy of tachyplesin III, colistin, and imipenem against a multiresistant Pseudomonas aeruginosa strain. In vitro experiments included MIC determination, time-kill, and synergy studies. For in vivo studies, a mouse model of sepsis has been used. The main outcome measures were bacterial lethality, quantitative blood cultures, and plasma levels of lipopolysaccharide, tumor necrosis factor alpha, and interleukin-6. The combination of tachyplesin III or colistin with imipenem showed in vitro synergistic interaction. A significant increase in efficacy was also observed in vivo: combination-treated groups had significantly lower levels of bacteremia than did groups treated with a single agent. Tachyplesin III combined with imipenem exhibited the highest efficacy on all main outcome measurements. These results highlight the potential usefulness of these combinations and provide therapeutic alternatives for serious infections caused by gram-negative bacteria in the coming years.

Original languageEnglish
Pages (from-to)2005-2010
Number of pages6
JournalAntimicrobial Agents and Chemotherapy
Volume51
Issue number6
DOIs
Publication statusPublished - Jun 2007

ASJC Scopus subject areas

  • Pharmacology (medical)

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