Efficiency of two different nine-loci short tandem repeat systems for DNA typing purposes

Lucia Sacchetti, Giuseppe Calcagno, Iolanda Coto, Nadia Tinto, Emilia Vuttariello, Francesco Salvatore

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: Genotyping based on short tandem repeat (STR) regions is widely used in human identification and parentage testing, in gene mapping studies, and as an approach to studies on the etiopathogenesis and diagnosis of hereditary diseases. We wished to study a new analytical approach that uses capillary electrophoresis and multicolor fluorescence in place of slab gel electrophoresis. Methods: We evaluated the efficiency for parentage and forensic purposes of the AmpFLSTR Profiler Plus(TM) typing kit that is used with the ABI Prism 310 Genetic Analyzer (System-2 STR), and that of a widely used panel of nine STRs analyzed with conventional slab-gel electrophoresis followed by radioactive detection (System-1 STR). System-2 STR, based on automated capillary electrophoresis and automated sizing of the alleles by Genotyper 2.0 software, was used to determine the allele frequency of the nine loci in 157 Caucasian subjects from southern Italy. On the basis of the data obtained, we submitted 40 trios to parentage testing. Results: A higher median probability of paternity attribution and power of exclusion were obtained with System-2 STR vs System-1 STR: respectively, 99.99% and 99.95% (P-12 for System-2 compared with 1.11 x 10-7 obtained with the other system. The internal standard and allelic ladder of the System-2 STR facilitated accurate and precise genotyping; furthermore, System-2 STR and was faster than the conventional System-1 STR. Conclusions: The System-2 STR allows rapid testing with higher probabilities of attribution and a higher power of exclusion than with the comparison method with slab-gel electrophoresis.

Original languageEnglish
Pages (from-to)178-183
Number of pages6
JournalClinical Chemistry
Volume45
Issue number2
Publication statusPublished - 1999

Fingerprint

DNA Fingerprinting
Microsatellite Repeats
DNA
Electrophoresis
Capillary electrophoresis
Gels
Capillary Electrophoresis
Testing
Forensic Anthropology
Inborn Genetic Diseases
Chromosome Mapping
Ladders
Prisms
Gene Frequency
Italy
Software
Genes
Fluorescence
Alleles

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Sacchetti, L., Calcagno, G., Coto, I., Tinto, N., Vuttariello, E., & Salvatore, F. (1999). Efficiency of two different nine-loci short tandem repeat systems for DNA typing purposes. Clinical Chemistry, 45(2), 178-183.

Efficiency of two different nine-loci short tandem repeat systems for DNA typing purposes. / Sacchetti, Lucia; Calcagno, Giuseppe; Coto, Iolanda; Tinto, Nadia; Vuttariello, Emilia; Salvatore, Francesco.

In: Clinical Chemistry, Vol. 45, No. 2, 1999, p. 178-183.

Research output: Contribution to journalArticle

Sacchetti, L, Calcagno, G, Coto, I, Tinto, N, Vuttariello, E & Salvatore, F 1999, 'Efficiency of two different nine-loci short tandem repeat systems for DNA typing purposes', Clinical Chemistry, vol. 45, no. 2, pp. 178-183.
Sacchetti, Lucia ; Calcagno, Giuseppe ; Coto, Iolanda ; Tinto, Nadia ; Vuttariello, Emilia ; Salvatore, Francesco. / Efficiency of two different nine-loci short tandem repeat systems for DNA typing purposes. In: Clinical Chemistry. 1999 ; Vol. 45, No. 2. pp. 178-183.
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