Efficient cDNA expression vectors for stable and transient expression of HLA-DR in transfected fibroblast and lymphoid cells

E. O. Long, S. Rosen-Bronson, D. R. Karp, M. Malnati, R. P. Sekaly, D. Jaraquemada

Research output: Contribution to journalArticle

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Abstract

cDNA expression vectors with several useful features were constructed. First, the long terminal repeat of Rous sarcoma virus was used as a promoter to obtain high levels of expression in various cells of human and mouse origin. Second, cis-linked expression units that confer resistance either to mycophenolic acid or the neomycin analog G418 were inserted to facilitate the isolation of transfected cells expressing the cDNA of interest. Third, by replicating in simian COS cells, these vectors can be used for efficient transient expression. cDNA fragments encoding the DRα or DRβ chains of human class II major histocompatibility complex antigens were inserted into these vectors and high levels of cell surface HLA-DR antigen were obtained after contransfection into mouse and human fibroblasts. These vectors were also successfully used to correct the inability of a class II-negative B cell line, derived from a patient with a congenital immunodeficiency, to present peptide antigen to DR-restricted T cells.

Original languageEnglish
Pages (from-to)229-235
Number of pages7
JournalHuman Immunology
Volume31
Issue number4
DOIs
Publication statusPublished - 1991

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HLA-DR Antigens
Complementary DNA
Fibroblasts
Lymphocytes
Mycophenolic Acid
Rous sarcoma virus
Neomycin
Terminal Repeat Sequences
Cell Separation
COS Cells
Histocompatibility Antigens Class II
Surface Antigens
Major Histocompatibility Complex
B-Lymphocytes
T-Lymphocytes
Antigens
Cell Line
Peptides

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Efficient cDNA expression vectors for stable and transient expression of HLA-DR in transfected fibroblast and lymphoid cells. / Long, E. O.; Rosen-Bronson, S.; Karp, D. R.; Malnati, M.; Sekaly, R. P.; Jaraquemada, D.

In: Human Immunology, Vol. 31, No. 4, 1991, p. 229-235.

Research output: Contribution to journalArticle

Long, E. O. ; Rosen-Bronson, S. ; Karp, D. R. ; Malnati, M. ; Sekaly, R. P. ; Jaraquemada, D. / Efficient cDNA expression vectors for stable and transient expression of HLA-DR in transfected fibroblast and lymphoid cells. In: Human Immunology. 1991 ; Vol. 31, No. 4. pp. 229-235.
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AU - Sekaly, R. P.

AU - Jaraquemada, D.

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