Efficient cultivation of neural stem cells with controlled delivery of FGF-2

U. Galderisi, G. Peluso, G. Di Bernardo, A. Calarco, M. D'Apolito, O. Petillo, M. Cipollaro, F. R. Fusco, M. A B Melone

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Neural stem cells (NSCs) raised the hope for cell-based therapies in human neurodevelopmental and neurodegenerative diseases. Current research strategies aim to isolate, enrich, and propagate homogeneous populations of neural stem cells. Unfortunately, several concerns with NSC cultures currently may limit their therapeutic promise. Exhaustion of growth factors and/or their uncontrolled release with burst and fall in their concentration may greatly affect the in vitro behavior of NSCs. In this context, we investigate whether a device containing heparan sulfate (HS), which is a co-factor in growth factor-mediated cell proliferation and differentiation, could potentiate and prolong the delivery of fibroblast growth factor-2 (FGF-2) and thus improve in vitro NSC cultivation. We demonstrated that NSCs cultivated in media with a controlled release of FGF-2 from a polyelectrolyte polymer showed a higher proliferation rate, and reduced apoptosis and senescence. In these experimental conditions NSCs preserve their stemness properties for a longer period of time compared with controls. Also of interest is that cell fate properties are conserved as well. The controlled release of FGF-2 reduced the level of oxidative stress and this is associated with a lower level of damaged DNA. This result may explain the reduced level of senescence and apoptosis in NSCs cultivated in the presence of hydrogel-releasing FGF-2.

Original languageEnglish
Pages (from-to)85-94
Number of pages10
JournalStem Cell Research
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Neural Stem Cells
Fibroblast Growth Factor 2
Intercellular Signaling Peptides and Proteins
Hope
Apoptosis
Heparitin Sulfate
Hydrogel
Cell- and Tissue-Based Therapy
Neurodegenerative Diseases
Cell Differentiation
Polymers
Oxidative Stress
Cell Culture Techniques
Cell Proliferation
Equipment and Supplies
DNA
Research

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Medicine(all)

Cite this

Galderisi, U., Peluso, G., Di Bernardo, G., Calarco, A., D'Apolito, M., Petillo, O., ... Melone, M. A. B. (2013). Efficient cultivation of neural stem cells with controlled delivery of FGF-2. Stem Cell Research, 10(1), 85-94. https://doi.org/10.1016/j.scr.2012.09.001

Efficient cultivation of neural stem cells with controlled delivery of FGF-2. / Galderisi, U.; Peluso, G.; Di Bernardo, G.; Calarco, A.; D'Apolito, M.; Petillo, O.; Cipollaro, M.; Fusco, F. R.; Melone, M. A B.

In: Stem Cell Research, Vol. 10, No. 1, 01.2013, p. 85-94.

Research output: Contribution to journalArticle

Galderisi, U, Peluso, G, Di Bernardo, G, Calarco, A, D'Apolito, M, Petillo, O, Cipollaro, M, Fusco, FR & Melone, MAB 2013, 'Efficient cultivation of neural stem cells with controlled delivery of FGF-2', Stem Cell Research, vol. 10, no. 1, pp. 85-94. https://doi.org/10.1016/j.scr.2012.09.001
Galderisi U, Peluso G, Di Bernardo G, Calarco A, D'Apolito M, Petillo O et al. Efficient cultivation of neural stem cells with controlled delivery of FGF-2. Stem Cell Research. 2013 Jan;10(1):85-94. https://doi.org/10.1016/j.scr.2012.09.001
Galderisi, U. ; Peluso, G. ; Di Bernardo, G. ; Calarco, A. ; D'Apolito, M. ; Petillo, O. ; Cipollaro, M. ; Fusco, F. R. ; Melone, M. A B. / Efficient cultivation of neural stem cells with controlled delivery of FGF-2. In: Stem Cell Research. 2013 ; Vol. 10, No. 1. pp. 85-94.
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