TY - JOUR
T1 - Efficient IgM assembly and secretion require the plasma cell induced endoplasmic reticulum protein pERp1
AU - Van Anken, Eelco
AU - Pena, Florentina
AU - Hafkemeijer, Nicole
AU - Christis, Chantal
AU - Romijn, Edwin P.
AU - Grauschopf, Ulla
AU - Oorschot, Viola M J
AU - Pertel, Thomas
AU - Engels, Sander
AU - Ora, Ari
AU - Lástun, Viorica
AU - Glockshuber, Rudi
AU - Klumperman, Judith
AU - Heck, Albert J R
AU - Luban, Jeremy
AU - Braakman, Ineke
PY - 2009/10/6
Y1 - 2009/10/6
N2 - Plasma cells daily secrete theirownmass in antibodies, which fold and assemble in the endoplasmic reticulum (ER). To reach these levels, cells require pERp1, a novel lymphocyte-specific small ER-resident protein, which attains expression levels as high as BiP when B cells differentiate into plasma cells. Although pERp1 has no homology with known ER proteins, it does contain a CXXC motif typical for oxidoreductases. In steady state, the CXXC cysteines are locked by two parallel disulfide bonds with a downstream C(X)6C motif, and pERp1 displays only modest oxidoreductase activity. pERp1 emerged as a dedicated folding factor for IgM, associating with both heavy and light chains and promoting assembly and secretion of mature IgM.
AB - Plasma cells daily secrete theirownmass in antibodies, which fold and assemble in the endoplasmic reticulum (ER). To reach these levels, cells require pERp1, a novel lymphocyte-specific small ER-resident protein, which attains expression levels as high as BiP when B cells differentiate into plasma cells. Although pERp1 has no homology with known ER proteins, it does contain a CXXC motif typical for oxidoreductases. In steady state, the CXXC cysteines are locked by two parallel disulfide bonds with a downstream C(X)6C motif, and pERp1 displays only modest oxidoreductase activity. pERp1 emerged as a dedicated folding factor for IgM, associating with both heavy and light chains and promoting assembly and secretion of mature IgM.
UR - http://www.scopus.com/inward/record.url?scp=70350130802&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70350130802&partnerID=8YFLogxK
U2 - 10.1073/pnas.0903036106
DO - 10.1073/pnas.0903036106
M3 - Article
C2 - 19805154
AN - SCOPUS:70350130802
VL - 106
SP - 17019
EP - 17024
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 40
ER -