Abstract
Human-immunodeficiency-virus (HIV)-based lentiviral vectors are a promising tool for in vivo gene therapy. Unlike Moloney-murine-leukaemia- based retroviruses (MLV), lentiviruses are believed to stably transduce quiescent (non-cycling) cells in various organs. No previous studies, however, have directly established the cell-cycle status of any transduced cell type at the time of vector administration in vivo. In vitro studies using wild-type HIV or HIV-based vectors have shown that, in some cases, cell-cycle activation is required for infection, even though cellular mitosis is not an absolute requirement for integration. Even if the block in reverse transcription is overcome in quiescent T cells, productive infection by HIV cannot be rescued in the absence of cell-cycle activation. The potential use of these vectors for gene therapy prompted our study, which establishes a cell-cycle requirement for efficient transduction of hepatocytes in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 49-52 |
| Number of pages | 4 |
| Journal | Nature Genetics |
| Volume | 24 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2000 |
Fingerprint
ASJC Scopus subject areas
- Genetics(clinical)
- Genetics
Cite this
Efficient lentiviral transduction of liver requires cell cycling in vivo. / Park, Frank; Ohashi, Kazuo; Chiu, Winnie; Naldini, Luigi; Mark A, Kay.
In: Nature Genetics, Vol. 24, No. 1, 2000, p. 49-52.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Efficient lentiviral transduction of liver requires cell cycling in vivo
AU - Park, Frank
AU - Ohashi, Kazuo
AU - Chiu, Winnie
AU - Naldini, Luigi
AU - Mark A, Kay
PY - 2000
Y1 - 2000
N2 - Human-immunodeficiency-virus (HIV)-based lentiviral vectors are a promising tool for in vivo gene therapy. Unlike Moloney-murine-leukaemia- based retroviruses (MLV), lentiviruses are believed to stably transduce quiescent (non-cycling) cells in various organs. No previous studies, however, have directly established the cell-cycle status of any transduced cell type at the time of vector administration in vivo. In vitro studies using wild-type HIV or HIV-based vectors have shown that, in some cases, cell-cycle activation is required for infection, even though cellular mitosis is not an absolute requirement for integration. Even if the block in reverse transcription is overcome in quiescent T cells, productive infection by HIV cannot be rescued in the absence of cell-cycle activation. The potential use of these vectors for gene therapy prompted our study, which establishes a cell-cycle requirement for efficient transduction of hepatocytes in vivo.
AB - Human-immunodeficiency-virus (HIV)-based lentiviral vectors are a promising tool for in vivo gene therapy. Unlike Moloney-murine-leukaemia- based retroviruses (MLV), lentiviruses are believed to stably transduce quiescent (non-cycling) cells in various organs. No previous studies, however, have directly established the cell-cycle status of any transduced cell type at the time of vector administration in vivo. In vitro studies using wild-type HIV or HIV-based vectors have shown that, in some cases, cell-cycle activation is required for infection, even though cellular mitosis is not an absolute requirement for integration. Even if the block in reverse transcription is overcome in quiescent T cells, productive infection by HIV cannot be rescued in the absence of cell-cycle activation. The potential use of these vectors for gene therapy prompted our study, which establishes a cell-cycle requirement for efficient transduction of hepatocytes in vivo.
UR - http://www.scopus.com/inward/record.url?scp=0033986340&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033986340&partnerID=8YFLogxK
U2 - 10.1038/71673
DO - 10.1038/71673
M3 - Article
C2 - 10615126
AN - SCOPUS:0033986340
VL - 24
SP - 49
EP - 52
JO - Nature Genetics
JF - Nature Genetics
SN - 1061-4036
IS - 1
ER -