Efficient Production of the Cyclosporin Metabolite AM1 By Rabbit Hepatic Enzymes

R. Paroni, I. Fermo, L. Iavarone, M. Del Puppo, L. De Angelis, M. Galli Kienle

Research output: Contribution to journalArticlepeer-review

Abstract

We describe here conditions for the in vitro preparation of the main metabolite of cyclosporin A, namely AM1, with rabbit liver enzymes. The compound was purified and was identified by comparison with the metabolite isolated from urine of rats treated with [3H]CsA and by its Mass-Spectrometry Fast Ion Bombardment (MS-FIB) and 1HNMR spectra. The procedure is simple and with the enzyme fraction derived from one rabbit liver 3-4 mg of the pure metabolite can be obtained.

Original languageEnglish
Pages (from-to)73-84
Number of pages12
JournalPharmacological Research
Volume28
Issue number1
DOIs
Publication statusPublished - Jul 1993

Keywords

  • HNMR
  • AM1
  • cyclosporin A
  • cyclosporin metabolites
  • Mass-spectrometry Fast Ion Bombardment (MS-FIB)

ASJC Scopus subject areas

  • Pharmacology

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