Efficient Production of the Cyclosporin Metabolite AM1 By Rabbit Hepatic Enzymes

R. Paroni, I. Fermo, L. Iavarone, M. Del Puppo, L. De Angelis, M. Galli Kienle

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We describe here conditions for the in vitro preparation of the main metabolite of cyclosporin A, namely AM1, with rabbit liver enzymes. The compound was purified and was identified by comparison with the metabolite isolated from urine of rats treated with [3H]CsA and by its Mass-Spectrometry Fast Ion Bombardment (MS-FIB) and 1HNMR spectra. The procedure is simple and with the enzyme fraction derived from one rabbit liver 3-4 mg of the pure metabolite can be obtained.

Original languageEnglish
Pages (from-to)73-84
Number of pages12
JournalPharmacological Research
Volume28
Issue number1
DOIs
Publication statusPublished - Jul 1993

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Cyclosporine
Rabbits
Liver
Enzymes
Mass Spectrometry
Urine
Ions
In Vitro Techniques

Keywords

  • HNMR
  • AM1
  • cyclosporin A
  • cyclosporin metabolites
  • Mass-spectrometry Fast Ion Bombardment (MS-FIB)

ASJC Scopus subject areas

  • Pharmacology

Cite this

Efficient Production of the Cyclosporin Metabolite AM1 By Rabbit Hepatic Enzymes. / Paroni, R.; Fermo, I.; Iavarone, L.; Del Puppo, M.; De Angelis, L.; Kienle, M. Galli.

In: Pharmacological Research, Vol. 28, No. 1, 07.1993, p. 73-84.

Research output: Contribution to journalArticle

Paroni, R, Fermo, I, Iavarone, L, Del Puppo, M, De Angelis, L & Kienle, MG 1993, 'Efficient Production of the Cyclosporin Metabolite AM1 By Rabbit Hepatic Enzymes', Pharmacological Research, vol. 28, no. 1, pp. 73-84. https://doi.org/10.1006/phrs.1993.1111
Paroni, R. ; Fermo, I. ; Iavarone, L. ; Del Puppo, M. ; De Angelis, L. ; Kienle, M. Galli. / Efficient Production of the Cyclosporin Metabolite AM1 By Rabbit Hepatic Enzymes. In: Pharmacological Research. 1993 ; Vol. 28, No. 1. pp. 73-84.
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