EGF regulates a complex pattern of gene expression and represses smooth muscle differentiation during the neurotypic conversion of the neural-crest-derived TC-1S cell line

Giuseppe Giannini, Edoardo Alesse, Lucia Di Marcotullio, Francesca Zazzeroni, Rita Gallo, Massimo Zani, Luigi Frati, Isabella Screpanti, Alberto Gulino

Research output: Contribution to journalArticlepeer-review

Abstract

EGF, known to sustain CNS neuronal progenitors, also promotes a neurotypic response in the thymic neural-crest-derived TC-1S cell line. We report here the use of TC-1S cells as a model to identify the genetic programs regulated during the neurotypic response induced by EGF and to isolate 23 EGF-responsive genes. Among them 5 represent novel cDNAs, while 18 are known genes, whose regulation by EGF is associated with the mitogenic or differentiating effects of the growth factor. The repression of smooth muscle α-actin and SM22α genes by EGF and their increase by TGFβ suggest that the TC-1S line includes neural crest multipotent cells whose smooth muscle differentiation is repressed upon EGF treatment and stimulated by TGFβ. Therefore, we identified a complex pattern of EGF-target genes and propose EGF as a novel signal able to recruit postmigratory neural-crest-derived cells along proliferation and cell lineage choice pathways.

Original languageEnglish
Pages (from-to)353-362
Number of pages10
JournalExperimental Cell Research
Volume264
Issue number2
DOIs
Publication statusPublished - Apr 1 2001

Keywords

  • Differential display
  • EGF
  • EGF-responsive genes
  • Neural crest
  • Smooth muscle
  • TGF

ASJC Scopus subject areas

  • Cell Biology

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