Abstract
Abstract The roles of the epidermal growth factor receptor (EGFR) in sebaceous glands remain poorly explored. We show that human sebocytes express EGFR and lower levels of ERBB2 and ERBB3, all receptors being downregulated after the induction of lipid synthesis. Nile red staining showed that siRNA-mediated downregulation of EGFR or ERBB3 increases lipid accumulation, whereas ERBB2 downregulation has no effect. Spectrometry confirmed induction of triglycerides after EGFR or ERBB3 downregulation and revealed induction of cholesteryl esters after downregulation of EGFR, ERBB2 or ERBB3. Thus, EGFR/ERBB receptors differentially modulate sebaceous lipogenesis, a key feature of sebaceous gland physiology and of several skin diseases.
| Original language | English |
|---|---|
| Article number | 37097 |
| Pages (from-to) | 1376-1382 |
| Number of pages | 7 |
| Journal | FEBS Letters |
| Volume | 589 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - May 22 2015 |
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Keywords
- Epidermal growth factor receptor
- ERBB
- Sebaceous gland
- Sebaceous lipogenesis
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Genetics
- Molecular Biology
- Structural Biology
- Medicine(all)
Cite this
EGFR/ERBB receptors differentially modulate sebaceous lipogenesis. / Dahlhoff, Maik; Camera, Emanuela; Ludovici, Matteo; Picardo, Mauro; Müller, Udo; Leonhardt, Heinrich; Zouboulis, Christos C.; Schneider, Marlon R.
In: FEBS Letters, Vol. 589, No. 12, 37097, 22.05.2015, p. 1376-1382.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - EGFR/ERBB receptors differentially modulate sebaceous lipogenesis
AU - Dahlhoff, Maik
AU - Camera, Emanuela
AU - Ludovici, Matteo
AU - Picardo, Mauro
AU - Müller, Udo
AU - Leonhardt, Heinrich
AU - Zouboulis, Christos C.
AU - Schneider, Marlon R.
PY - 2015/5/22
Y1 - 2015/5/22
N2 - Abstract The roles of the epidermal growth factor receptor (EGFR) in sebaceous glands remain poorly explored. We show that human sebocytes express EGFR and lower levels of ERBB2 and ERBB3, all receptors being downregulated after the induction of lipid synthesis. Nile red staining showed that siRNA-mediated downregulation of EGFR or ERBB3 increases lipid accumulation, whereas ERBB2 downregulation has no effect. Spectrometry confirmed induction of triglycerides after EGFR or ERBB3 downregulation and revealed induction of cholesteryl esters after downregulation of EGFR, ERBB2 or ERBB3. Thus, EGFR/ERBB receptors differentially modulate sebaceous lipogenesis, a key feature of sebaceous gland physiology and of several skin diseases.
AB - Abstract The roles of the epidermal growth factor receptor (EGFR) in sebaceous glands remain poorly explored. We show that human sebocytes express EGFR and lower levels of ERBB2 and ERBB3, all receptors being downregulated after the induction of lipid synthesis. Nile red staining showed that siRNA-mediated downregulation of EGFR or ERBB3 increases lipid accumulation, whereas ERBB2 downregulation has no effect. Spectrometry confirmed induction of triglycerides after EGFR or ERBB3 downregulation and revealed induction of cholesteryl esters after downregulation of EGFR, ERBB2 or ERBB3. Thus, EGFR/ERBB receptors differentially modulate sebaceous lipogenesis, a key feature of sebaceous gland physiology and of several skin diseases.
KW - Epidermal growth factor receptor
KW - ERBB
KW - Sebaceous gland
KW - Sebaceous lipogenesis
UR - http://www.scopus.com/inward/record.url?scp=84937762868&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84937762868&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2015.04.003
DO - 10.1016/j.febslet.2015.04.003
M3 - Article
VL - 589
SP - 1376
EP - 1382
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 12
M1 - 37097
ER -