Electrical and ionic mechanisms of early reperfusion arrhythmias in sheep cardiac Purkinje's fibers

Pasquale Abete, Roberto Bernabei, Marco di Gennaro, Giovanni Iacono, Franco Rengo, Pierugo Carbonin, Mario Vassalle

Research output: Contribution to journalArticle

Abstract

The mechanisms of induction of early reperfusion arrhythmias were studied in sheep cardiac Purkinje's fibers superfused in vitro. Transmembrane potentials, intracellular sodium activity (ai Na), and contractile force were recorded. Stoppage of the flow of Tyrode's solution (ischemia) for 1 hour initially decreased slightly ai Na (-0.57 mmol -7.2%), increased the action potential amplitude (+6.1%) and duration (+7.8%), and decreased diastolic depolarization slope (-45.2%). As the ischemia continued, ai Na increased progressively (to 12.53 mmol, +56.2%), whereas force peaked (+395%) after about 30 minutes and then began to decrease. By the end of ischemia, there was a decrease in action potential amplitude (-14.9%) and duration (-39.6%), whereas diastolic depolarization slope reincreased again almost to control value (-7%). When the flow of Tyrode's solution was resumed (reperfusion), force markedly increased (+211.1%) and oscillatory potentials initiated arrhythmias (extrasystoles and repetitive fast discharge) in 64% of tests. Force and ai Na decreased relatively rapidly. The arrhythmias initiated after 58.4 ± 1.8 seconds of reperfusion and lasted 101.5 ± 3.2 seconds. When [Na]o was increased by +19.2%, reperfusion arrhythmias occurred after only 30 minutes of ischemia. Thus, in Purkinje's fibers superfused in vitro, early reperfusion arrhythmias are induced by oscillatory potentials caused by calcium overload, which is enhanced by the increase in ai Na during ischemia.

Original languageEnglish
Pages (from-to)199-212
Number of pages14
JournalJournal of Electrocardiology
Volume21
Issue number3
DOIs
Publication statusPublished - 1988

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Purkinje Fibers
Reperfusion
Cardiac Arrhythmias
Sheep
Ischemia
Action Potentials
Premature Cardiac Complexes
Membrane Potentials
Sodium
Calcium

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Abete, P., Bernabei, R., di Gennaro, M., Iacono, G., Rengo, F., Carbonin, P., & Vassalle, M. (1988). Electrical and ionic mechanisms of early reperfusion arrhythmias in sheep cardiac Purkinje's fibers. Journal of Electrocardiology, 21(3), 199-212. https://doi.org/10.1016/0022-0736(88)90094-5

Electrical and ionic mechanisms of early reperfusion arrhythmias in sheep cardiac Purkinje's fibers. / Abete, Pasquale; Bernabei, Roberto; di Gennaro, Marco; Iacono, Giovanni; Rengo, Franco; Carbonin, Pierugo; Vassalle, Mario.

In: Journal of Electrocardiology, Vol. 21, No. 3, 1988, p. 199-212.

Research output: Contribution to journalArticle

Abete, P, Bernabei, R, di Gennaro, M, Iacono, G, Rengo, F, Carbonin, P & Vassalle, M 1988, 'Electrical and ionic mechanisms of early reperfusion arrhythmias in sheep cardiac Purkinje's fibers', Journal of Electrocardiology, vol. 21, no. 3, pp. 199-212. https://doi.org/10.1016/0022-0736(88)90094-5
Abete, Pasquale ; Bernabei, Roberto ; di Gennaro, Marco ; Iacono, Giovanni ; Rengo, Franco ; Carbonin, Pierugo ; Vassalle, Mario. / Electrical and ionic mechanisms of early reperfusion arrhythmias in sheep cardiac Purkinje's fibers. In: Journal of Electrocardiology. 1988 ; Vol. 21, No. 3. pp. 199-212.
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abstract = "The mechanisms of induction of early reperfusion arrhythmias were studied in sheep cardiac Purkinje's fibers superfused in vitro. Transmembrane potentials, intracellular sodium activity (ai Na), and contractile force were recorded. Stoppage of the flow of Tyrode's solution (ischemia) for 1 hour initially decreased slightly ai Na (-0.57 mmol -7.2{\%}), increased the action potential amplitude (+6.1{\%}) and duration (+7.8{\%}), and decreased diastolic depolarization slope (-45.2{\%}). As the ischemia continued, ai Na increased progressively (to 12.53 mmol, +56.2{\%}), whereas force peaked (+395{\%}) after about 30 minutes and then began to decrease. By the end of ischemia, there was a decrease in action potential amplitude (-14.9{\%}) and duration (-39.6{\%}), whereas diastolic depolarization slope reincreased again almost to control value (-7{\%}). When the flow of Tyrode's solution was resumed (reperfusion), force markedly increased (+211.1{\%}) and oscillatory potentials initiated arrhythmias (extrasystoles and repetitive fast discharge) in 64{\%} of tests. Force and ai Na decreased relatively rapidly. The arrhythmias initiated after 58.4 ± 1.8 seconds of reperfusion and lasted 101.5 ± 3.2 seconds. When [Na]o was increased by +19.2{\%}, reperfusion arrhythmias occurred after only 30 minutes of ischemia. Thus, in Purkinje's fibers superfused in vitro, early reperfusion arrhythmias are induced by oscillatory potentials caused by calcium overload, which is enhanced by the increase in ai Na during ischemia.",
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