Electrical and mechanical actions of magnesium sulfate during ischemia and reperfusion on isolated perfused rat heart

N. Ferrara, G. Longobardi, P. Abete, D. Leosco, G. Monaco, P. Caccese, D. Vitale, B. Tuccillo, F. Rengo

Research output: Contribution to journalArticle

Abstract

Experimental and clinical studies have shown the action of magnesium salts on myocardial tissue and its antiarrhythmic action on digitalis-induced ventricular arrhythmias. We have evaluated the effects of magnesium sulfate (2.5 mM) on heart rate (HR), coronary flow rate (CFR), left ventricular systolic pressure (LVSP), dP/dt max and voltage epicardial electrogram (VEE) during ischemia and reperfusion in isolated perfused rat heart. Forty-five male rats were randomized into 3 groups. In the control group, after a 15 min period of stabilization, hearts were perfused by ischemic perfusion for 30 min and then reperfused. In group I, magnesium sulfate was given after stabilization and before the ischemic period. In group II, calcium concentration was increased by 0.5 mM and magnesium sulfate was given after stabilization and before the ischemic period. In the control group, we observed during ischemia a reduction in HR, CFR, LVSP, dP/dt max and an increase in VEE. In group I, after the administration of magnesium sulfate, we noted a decrease in HR, LVSP, dP/dt max, VEE and during the ischemic period there was no difference in these parameters with respect to the control group. In group II, the increase of extracellular calcium concentration caused an increase in LVSP, dP/dt max and the administration of magnesium sulfate abolished these effects, bringing the values back to basal values. Reperfusion provoked ventricular arrhythmias in 11/15 and 12/15 hearts in the control group and group II respectively, and only 3/15 in group I (p <0.005 group I vs control and group II). Thus, our results demonstrated that magnesium sulfate has varied effects on electrical and mechanical parameters and prevents reperfusion arrhythmias according to the hypothesis of a calcium-antagonist mechanism of magnesium.

Original languageEnglish
Pages (from-to)84-96
Number of pages13
JournalArchives Internationales de Pharmacodynamie et de Therapie
Volume293
Issue number1
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Pharmacology

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    Ferrara, N., Longobardi, G., Abete, P., Leosco, D., Monaco, G., Caccese, P., Vitale, D., Tuccillo, B., & Rengo, F. (1988). Electrical and mechanical actions of magnesium sulfate during ischemia and reperfusion on isolated perfused rat heart. Archives Internationales de Pharmacodynamie et de Therapie, 293(1), 84-96.