The mitochondrial reactive oxygen species (ROS) production is a crucial issue that has been involved in a variety of physio-pathological processes and aging. However, at the present measurements of mitochondrial ROS production is controversial. Indeed, the available tools are poorly specific and not direct. Now taking advantage of the progress in microelectrode production it has been possible to approach the specific and direct measurement of O 2 species in a suspension of isolated mitochondria. Such electrochemical determinations confirm active H 2 O 2 release by mitochondria above the scavenging activity and reveal a significant role of ATP synthase activation in the control of mitochondrial ROS production. These findings offer a new explanation for the effects of mitochondrial respiration on oxidative stress. Regardless the damaging effect of ROS that has been already associated to intense mitochondrial work during exercise in muscle for example, H 2 O 2 spikes may represent specific cellular signal. The crosstalk between the mitochondrion and the cell is indeed an emergent issue. About this argument our observation discloses a novel basic mechanism of mitochondrial ROS production and highlights the involvement of intracellular redox balance on health.
|Title of host publication||Hydrogen Peroxide: Detection, Applications and Health Implications|
|Publisher||Nova Science Publishers, Inc.|
|Number of pages||14|
|Publication status||Published - Jan 2013|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)