Electrochemical detection of H2O2 formation in isolated mitochondria

Stefania Rapino, Raluca Marcu, Francesco Paolucci, Marco Giorgio

Research output: Chapter in Book/Report/Conference proceedingChapter


Mitochondrial respiration produces both complete and partially reduced oxygen species that are involved in physiological and pathological processes. Indeed, unspecific oxidative damage induced by excessive mitochondrial reactive oxygen species (ROS) plays a role in aging and several diseases, whereas low amounts of ROS act in physiological signaling processes. The exact molecular species, the rate, and the conditions of mitochondrial ROS release are not clearly evaluable by current methods based on oxidation sensitive markers. Recently, electrochemical analysis of biological samples has improved. Following latest methodology, we implemented a novel electrochemical assay for the investigation of aerobic metabolism in isolated mitochondria through simultaneous measurement of O2 consumption and H2O 2 production. Our experiments confirm active H2O 2 production by respiring mouse liver mitochondria and show that ATP synthase activation increases the rate of H2O2, suggesting that state 3 mitochondria might induce the cell through oxidative signals.

Original languageEnglish
Title of host publicationMethods in Enzymology
Number of pages12
Publication statusPublished - 2013

Publication series

NameMethods in Enzymology
ISSN (Print)00766879
ISSN (Electronic)15577988


  • Electrochemistry
  • Hydrogen peroxide
  • Mitochondria
  • Respiratory chain

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Medicine(all)


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