TY - JOUR
T1 - Electrochemical study of hydrogen peroxide formation in isolated mitochondria
AU - Marcu, Raluca
AU - Rapino, Stefania
AU - Trinei, Mirella
AU - Valenti, Giovanni
AU - Marcaccio, Massimo
AU - Pelicci, Pier Giuseppe
AU - Paolucci, Francesco
AU - Giorgio, Marco
PY - 2012/6
Y1 - 2012/6
N2 - Mitochondrial respiration generates reactive oxygen species that are involved in physiological and pathological processes. The majority of methods, with exception of electron paramagnetic resonance, used to evaluate the identity, the rate and the conditions of the reactive oxygen species produced by mitochondria, are mainly based on oxidation sensitive markers. Following latest electrochemical methodology, we implemented a novel electrochemical assay for the investigation of aerobic metabolism in preparations of isolated mitochondria through simultaneous measurement of O 2 consumption and reactive species production. This electrochemical assay reveals active H 2O 2 production by respiring mouse liver mitochondria, and shows that ATP synthase activation and moderate depolarization increase the rate of H 2O 2 formation, suggesting that ATP synthesizing (state 3) mitochondria might contribute to oxidative stress or signaling.
AB - Mitochondrial respiration generates reactive oxygen species that are involved in physiological and pathological processes. The majority of methods, with exception of electron paramagnetic resonance, used to evaluate the identity, the rate and the conditions of the reactive oxygen species produced by mitochondria, are mainly based on oxidation sensitive markers. Following latest electrochemical methodology, we implemented a novel electrochemical assay for the investigation of aerobic metabolism in preparations of isolated mitochondria through simultaneous measurement of O 2 consumption and reactive species production. This electrochemical assay reveals active H 2O 2 production by respiring mouse liver mitochondria, and shows that ATP synthase activation and moderate depolarization increase the rate of H 2O 2 formation, suggesting that ATP synthesizing (state 3) mitochondria might contribute to oxidative stress or signaling.
KW - Amperometry
KW - Hydrogen peroxide
KW - Mitochondria
KW - Platinized carbon fiber microelectrodes
UR - http://www.scopus.com/inward/record.url?scp=84857373542&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84857373542&partnerID=8YFLogxK
U2 - 10.1016/j.bioelechem.2011.11.005
DO - 10.1016/j.bioelechem.2011.11.005
M3 - Article
C2 - 22197548
AN - SCOPUS:84857373542
VL - 85
SP - 21
EP - 28
JO - Bioelectrochemistry
JF - Bioelectrochemistry
SN - 1567-5394
ER -