Electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto titanium fiber-mesh scaffolds

Lorenzo Fassina, Enrica Saino, Livia Visai, Giulia Silvani, Maria Gabriella Cusella De Angelis, Giuliano Mazzini, Francesco Benazzo, Giovanni Magenes

Research output: Contribution to journalArticle


The surface properties of a biomaterial are fundamental to determine the response of the host tissue. In the present study, we have followed a particular biomimetic strategy where electromagnetically stimulated SAOS-2 human osteoblasts proliferated and built a calcified extracellular matrix on a titanium fiber-mesh surface. In comparison with control conditions, the electromagnetic stimulation (magnetic field intensity, 2 mT; frequency, 75 Hz) caused higher cell proliferation and increased surface coating with type-I collagen, decorin, and osteopontin (9.8-fold, 11.3-fold, and 9.5-fold, respectively). Reverse transcriptase-polymerase analysis revealed the electromagnetically upregulated transcription specific for the foregoing matrix proteins and for the growth factor TGF-β1. The immunofluorescence of type-I collagen, decorin, and osteopontin showed their colocalization in the cell-rich areas. The use of an electromagnetic bioreactor aimed at obtaining the surface modification of the biocompatible metallic scaffold in terms of cell colonization and coating with calcified extracellular matrix. The superficially modified biomaterial could be used, in clinical applications, as an implant for bone repair.

Original languageEnglish
Pages (from-to)750-759
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Issue number3
Publication statusPublished - Dec 1 2008


  • Biomimetics
  • Calcified extracellular matrix
  • Cell proliferation
  • Decorin
  • Electromagnetic stimulation
  • Osteoblast
  • Osteopontin
  • Surface modification
  • Type-I collagen

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys
  • Medicine(all)

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