Electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto titanium fiber-mesh scaffolds

Lorenzo Fassina, Enrica Saino, Livia Visai, Giulia Silvani, Maria Gabriella Cusella De Angelis, Giuliano Mazzini, Francesco Benazzo, Giovanni Magenes

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The surface properties of a biomaterial are fundamental to determine the response of the host tissue. In the present study, we have followed a particular biomimetic strategy where electromagnetically stimulated SAOS-2 human osteoblasts proliferated and built a calcified extracellular matrix on a titanium fiber-mesh surface. In comparison with control conditions, the electromagnetic stimulation (magnetic field intensity, 2 mT; frequency, 75 Hz) caused higher cell proliferation and increased surface coating with type-I collagen, decorin, and osteopontin (9.8-fold, 11.3-fold, and 9.5-fold, respectively). Reverse transcriptase-polymerase analysis revealed the electromagnetically upregulated transcription specific for the foregoing matrix proteins and for the growth factor TGF-β1. The immunofluorescence of type-I collagen, decorin, and osteopontin showed their colocalization in the cell-rich areas. The use of an electromagnetic bioreactor aimed at obtaining the surface modification of the biocompatible metallic scaffold in terms of cell colonization and coating with calcified extracellular matrix. The superficially modified biomaterial could be used, in clinical applications, as an implant for bone repair.

Original languageEnglish
Pages (from-to)750-759
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume87
Issue number3
DOIs
Publication statusPublished - Dec 1 2008

Fingerprint

Decorin
Osteopontin
Electromagnetic Phenomena
Osteoblasts
Biocompatible Materials
Collagen Type I
Cell culture
Collagen
Biomaterials
Scaffolds
Extracellular Matrix
Titanium
Coatings
Biomimetics
Surface Properties
Fibers
RNA-Directed DNA Polymerase
Cell proliferation
Bioreactors
Magnetic Fields

Keywords

  • Biomimetics
  • Calcified extracellular matrix
  • Cell proliferation
  • Decorin
  • Electromagnetic stimulation
  • Osteoblast
  • Osteopontin
  • Surface modification
  • Type-I collagen

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys
  • Medicine(all)

Cite this

Electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto titanium fiber-mesh scaffolds. / Fassina, Lorenzo; Saino, Enrica; Visai, Livia; Silvani, Giulia; De Angelis, Maria Gabriella Cusella; Mazzini, Giuliano; Benazzo, Francesco; Magenes, Giovanni.

In: Journal of Biomedical Materials Research - Part A, Vol. 87, No. 3, 01.12.2008, p. 750-759.

Research output: Contribution to journalArticle

Fassina, Lorenzo ; Saino, Enrica ; Visai, Livia ; Silvani, Giulia ; De Angelis, Maria Gabriella Cusella ; Mazzini, Giuliano ; Benazzo, Francesco ; Magenes, Giovanni. / Electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto titanium fiber-mesh scaffolds. In: Journal of Biomedical Materials Research - Part A. 2008 ; Vol. 87, No. 3. pp. 750-759.
@article{e56b1b2050e94936a154d10f8b5c1e85,
title = "Electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto titanium fiber-mesh scaffolds",
abstract = "The surface properties of a biomaterial are fundamental to determine the response of the host tissue. In the present study, we have followed a particular biomimetic strategy where electromagnetically stimulated SAOS-2 human osteoblasts proliferated and built a calcified extracellular matrix on a titanium fiber-mesh surface. In comparison with control conditions, the electromagnetic stimulation (magnetic field intensity, 2 mT; frequency, 75 Hz) caused higher cell proliferation and increased surface coating with type-I collagen, decorin, and osteopontin (9.8-fold, 11.3-fold, and 9.5-fold, respectively). Reverse transcriptase-polymerase analysis revealed the electromagnetically upregulated transcription specific for the foregoing matrix proteins and for the growth factor TGF-β1. The immunofluorescence of type-I collagen, decorin, and osteopontin showed their colocalization in the cell-rich areas. The use of an electromagnetic bioreactor aimed at obtaining the surface modification of the biocompatible metallic scaffold in terms of cell colonization and coating with calcified extracellular matrix. The superficially modified biomaterial could be used, in clinical applications, as an implant for bone repair.",
keywords = "Biomimetics, Calcified extracellular matrix, Cell proliferation, Decorin, Electromagnetic stimulation, Osteoblast, Osteopontin, Surface modification, Type-I collagen",
author = "Lorenzo Fassina and Enrica Saino and Livia Visai and Giulia Silvani and {De Angelis}, {Maria Gabriella Cusella} and Giuliano Mazzini and Francesco Benazzo and Giovanni Magenes",
year = "2008",
month = "12",
day = "1",
doi = "10.1002/jbm.a.31827",
language = "English",
volume = "87",
pages = "750--759",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Electromagnetic enhancement of a culture of human SAOS-2 osteoblasts seeded onto titanium fiber-mesh scaffolds

AU - Fassina, Lorenzo

AU - Saino, Enrica

AU - Visai, Livia

AU - Silvani, Giulia

AU - De Angelis, Maria Gabriella Cusella

AU - Mazzini, Giuliano

AU - Benazzo, Francesco

AU - Magenes, Giovanni

PY - 2008/12/1

Y1 - 2008/12/1

N2 - The surface properties of a biomaterial are fundamental to determine the response of the host tissue. In the present study, we have followed a particular biomimetic strategy where electromagnetically stimulated SAOS-2 human osteoblasts proliferated and built a calcified extracellular matrix on a titanium fiber-mesh surface. In comparison with control conditions, the electromagnetic stimulation (magnetic field intensity, 2 mT; frequency, 75 Hz) caused higher cell proliferation and increased surface coating with type-I collagen, decorin, and osteopontin (9.8-fold, 11.3-fold, and 9.5-fold, respectively). Reverse transcriptase-polymerase analysis revealed the electromagnetically upregulated transcription specific for the foregoing matrix proteins and for the growth factor TGF-β1. The immunofluorescence of type-I collagen, decorin, and osteopontin showed their colocalization in the cell-rich areas. The use of an electromagnetic bioreactor aimed at obtaining the surface modification of the biocompatible metallic scaffold in terms of cell colonization and coating with calcified extracellular matrix. The superficially modified biomaterial could be used, in clinical applications, as an implant for bone repair.

AB - The surface properties of a biomaterial are fundamental to determine the response of the host tissue. In the present study, we have followed a particular biomimetic strategy where electromagnetically stimulated SAOS-2 human osteoblasts proliferated and built a calcified extracellular matrix on a titanium fiber-mesh surface. In comparison with control conditions, the electromagnetic stimulation (magnetic field intensity, 2 mT; frequency, 75 Hz) caused higher cell proliferation and increased surface coating with type-I collagen, decorin, and osteopontin (9.8-fold, 11.3-fold, and 9.5-fold, respectively). Reverse transcriptase-polymerase analysis revealed the electromagnetically upregulated transcription specific for the foregoing matrix proteins and for the growth factor TGF-β1. The immunofluorescence of type-I collagen, decorin, and osteopontin showed their colocalization in the cell-rich areas. The use of an electromagnetic bioreactor aimed at obtaining the surface modification of the biocompatible metallic scaffold in terms of cell colonization and coating with calcified extracellular matrix. The superficially modified biomaterial could be used, in clinical applications, as an implant for bone repair.

KW - Biomimetics

KW - Calcified extracellular matrix

KW - Cell proliferation

KW - Decorin

KW - Electromagnetic stimulation

KW - Osteoblast

KW - Osteopontin

KW - Surface modification

KW - Type-I collagen

UR - http://www.scopus.com/inward/record.url?scp=56349084755&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=56349084755&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.31827

DO - 10.1002/jbm.a.31827

M3 - Article

VL - 87

SP - 750

EP - 759

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 1549-3296

IS - 3

ER -