Electromagnetic fields (EMFs) and adenosine receptors modulate prostaglandin E 2 and cytokine release in human osteoarthritic synovial fibroblasts

A. Ongaro, K. Varani, F. F. Masieri, A. Pellati, L. Massari, R. Cadossi, F. Vincenzi, P. A. Borea, M. Fini, A. Caruso, M. De Mattei

Research output: Contribution to journalArticle

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Abstract

Synovial fibroblasts (SFs) contribute to the development of osteoarthritis (OA) by the secretion of a wide range of pro-inflammatory mediators, including cytokines and lipid mediators of inflammation. Previous studies suggest that electromagnetic fields (EMFs) may represent a potential therapeutic approach to limit cartilage degradation and control inflammation associated to OA, and that they may act through the adenosine pathway. Therefore, we investigated whether EMFs might modulate inflammatory activities of human SFs from OA patients (OASFs) treated with interleukin-1β (IL-1β), and the possible involvement of adenosine receptors (ARs) in mediating EMF effects. EMF exposure induced a selective increase in A 2A and A 3 ARs. These increases were associated to changes in cAMP levels, indicating that ARs were functionally active also in EMF-exposed cells. Functional data obtained in the presence of selective A 2A and A 3 adenosine agonists and antagonists showed that EMFs inhibit the release of prostaglandin E 2 (PGE 2) and the proinflammatory cytokines interleukin-6 (IL-6) and interleukin-8 (IL-8), while stimulating the release of interleukin-10 (IL-10), an antinflammatory cytokine. These effects seem to be mediated by the EMF-induced upregulation of A 2A and A 3 ARs. No effects of EMFs or ARs have been observed on matrix degrading enzyme production. In conclusion, this study shows that EMFs display anti-inflammatory effects in human OASFs, and that these EMF-induced effects are in part mediated by the adenosine pathway, specifically by the A 2A and A 3 AR activation. Taken together, these results open new clinical perspectives to the control of inflammation associated to joint diseases.

Original languageEnglish
Pages (from-to)2461-2469
Number of pages9
JournalJournal of Cellular Physiology
Volume227
Issue number6
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Purinergic P1 Receptors
Electromagnetic Fields
Fibroblasts
Prostaglandins E
Electromagnetic fields
Cytokines
Adenosine A2A Receptors
Adenosine
Osteoarthritis
Electromagnetic field effects
Inflammation Mediators
Inflammation
Cartilage
Joint Diseases
Interleukin-8
Interleukin-1
Interleukin-10
Human Activities
Interleukin-6
Anti-Inflammatory Agents

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Electromagnetic fields (EMFs) and adenosine receptors modulate prostaglandin E 2 and cytokine release in human osteoarthritic synovial fibroblasts. / Ongaro, A.; Varani, K.; Masieri, F. F.; Pellati, A.; Massari, L.; Cadossi, R.; Vincenzi, F.; Borea, P. A.; Fini, M.; Caruso, A.; De Mattei, M.

In: Journal of Cellular Physiology, Vol. 227, No. 6, 06.2012, p. 2461-2469.

Research output: Contribution to journalArticle

Ongaro, A, Varani, K, Masieri, FF, Pellati, A, Massari, L, Cadossi, R, Vincenzi, F, Borea, PA, Fini, M, Caruso, A & De Mattei, M 2012, 'Electromagnetic fields (EMFs) and adenosine receptors modulate prostaglandin E 2 and cytokine release in human osteoarthritic synovial fibroblasts', Journal of Cellular Physiology, vol. 227, no. 6, pp. 2461-2469. https://doi.org/10.1002/jcp.22981
Ongaro, A. ; Varani, K. ; Masieri, F. F. ; Pellati, A. ; Massari, L. ; Cadossi, R. ; Vincenzi, F. ; Borea, P. A. ; Fini, M. ; Caruso, A. ; De Mattei, M. / Electromagnetic fields (EMFs) and adenosine receptors modulate prostaglandin E 2 and cytokine release in human osteoarthritic synovial fibroblasts. In: Journal of Cellular Physiology. 2012 ; Vol. 227, No. 6. pp. 2461-2469.
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AU - Varani, K.

AU - Masieri, F. F.

AU - Pellati, A.

AU - Massari, L.

AU - Cadossi, R.

AU - Vincenzi, F.

AU - Borea, P. A.

AU - Fini, M.

AU - Caruso, A.

AU - De Mattei, M.

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