Electromagnetically stimulated SAOS-2 osteoblasts inside a porous Hy-droxyapatite scaffold in vitro

L. Fassina, L. Visai, E. Saino, M. S. Sbarra, G. Magenes

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Several studies suggest that the modification of a biomaterial surface play an important role in bone tissue engineering. In this study we have followed a biomimetic strategy where electromagnetically stimulated SAOS-2 osteoblasts, from a human osteosarcoma cell line, proliferated and built their extracellular matrix inside a porous hydroxyapatite scaffold. In comparison with control static conditions, the electromagnetic stimulus (magnetic field, 2 mT; frequency, 75 Hz) increased the cell proliferation and the production of bone proteins (decorin, osteocalcin, osteopontin, type-I collagen, and type-III collagen), with a consequent surface coating of the scaffold. The physical stimulus was aimed at obtaining a biomimetic modification of the internal porous surface of the hydroxyapatite scaffold. The cell-biomaterial construct could be used as an implant for bone repair in clinical applications.

Original languageEnglish
Title of host publication12th International Conference on Fracture 2009, ICF-12
Pages3536-3545
Number of pages10
Volume5
Publication statusPublished - 2009
Event12th International Conference on Fracture 2009, ICF-12 - Ottawa, ON, Canada
Duration: Jul 12 2009Jul 17 2009

Other

Other12th International Conference on Fracture 2009, ICF-12
CountryCanada
CityOttawa, ON
Period7/12/097/17/09

ASJC Scopus subject areas

  • Geotechnical Engineering and Engineering Geology

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    Fassina, L., Visai, L., Saino, E., Sbarra, M. S., & Magenes, G. (2009). Electromagnetically stimulated SAOS-2 osteoblasts inside a porous Hy-droxyapatite scaffold in vitro. In 12th International Conference on Fracture 2009, ICF-12 (Vol. 5, pp. 3536-3545)