Electron microscopy analysis of early localization of cisplatin in ovarian carcinoma cells

Giovanni L. Beretta, Sabina C. Righetti, Luciano Lombardi, Franco Zunino, Paola Perego

Research output: Contribution to journalArticle

Abstract

Taking advantage of the electron-dense nature of platinum, in this study the authors used an electron microscopy approach to investigate the cellular localization of cisplatin in an ovarian carcinoma cell line. Platinum spots were detected in contact with the plasma membrane and the nuclear envelope as well as in the cytoplasm and nuclear matrices. Contact with the plasma membrane was through a single blunt contact or spanning through the membrane. No sequestration in intracellular vescicles was observed, thereby supporting that phagocytosis and receptor-mediated endocytosis were not occurring. A molecular analysis indicated lack of expression of aquaporin 9, thus excluding its involvement in the membrane translocation of cisplatin. The present data suggest that cisplatin rapidly accumulates in the cell through endocytosis-independent membrane translocation and are consistent with passive diffusion.

Original languageEnglish
Pages (from-to)331-334
Number of pages4
JournalUltrastructural Pathology
Volume26
Issue number5
DOIs
Publication statusPublished - Sep 2002

Keywords

  • Cisplatin
  • Localization
  • Ovarian carcinoma cells

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Instrumentation
  • Structural Biology

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