Electron paramagnetic resonance studies on the fluidity and surface dynamics of egg phosphatidylcholine vesicles containing gangliosides

E. Bertoli, M. Masserini, S. Sonnino, R. Ghidoni, B. Cestaro, G. Tettamanti

Research output: Contribution to journalArticle

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Abstract

The influence of different gangliosides (G(M1), G(D1a), G(T1b)) on the fluidity and surface dynamics of phosphatidylcholine small unilamellar vesicles was studied by electron paramagnetic resonance. 5- and 16-nitroxystearic acid, sounding respectively the region close to the surface and that close to the hydrophobic core of the vesicle, were employed as spin-label probes. The signals released by 5-nitroxystearic acid showed that the presence of gangliosides reduced the mobility of the hydrocarbon chains around the probe. The effect increased by increasing ganglioside concentration, and diminished from G(M1) to G(D1a) and G(T1b). The decrease of membrane fluidity was also monitored by the 16-nitroxystearic acid probe. On addition of Ca2+ the fluidity of ganglioside-containing vesicles (as signalled by the 5-nitroxystearic acid probe) promptly decreased, thereafter returning slowly to the original value. It is suggested that gangliosides cause strong side-side head group interactions on the bilayer surface - between ganglioside oligosaccharide chains and between ganglioside and phosphatidylcholine polar portions - which lead the lipid chains to assembly in a more rigid fashion. The influence of Ca2+ is interpreted as due to lateral phase separation in the vesicle membrane. This phenomenon can be related to the formation or stabilization of ganglioside clusters on the vesicle surface.

Original languageEnglish
Pages (from-to)196-202
Number of pages7
JournalBBA - General Subjects
Volume647
Issue number2
DOIs
Publication statusPublished - 1982

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Fluidity
Gangliosides
Electron Spin Resonance Spectroscopy
Phosphatidylcholines
Ovum
Paramagnetic resonance
Membranes
G(M1) Ganglioside
Spin Labels
Unilamellar Liposomes
Membrane Fluidity
Hydrocarbons
Oligosaccharides
Phase separation
Stabilization
Lipids
5-doxylstearic acid

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Electron paramagnetic resonance studies on the fluidity and surface dynamics of egg phosphatidylcholine vesicles containing gangliosides. / Bertoli, E.; Masserini, M.; Sonnino, S.; Ghidoni, R.; Cestaro, B.; Tettamanti, G.

In: BBA - General Subjects, Vol. 647, No. 2, 1982, p. 196-202.

Research output: Contribution to journalArticle

Bertoli, E. ; Masserini, M. ; Sonnino, S. ; Ghidoni, R. ; Cestaro, B. ; Tettamanti, G. / Electron paramagnetic resonance studies on the fluidity and surface dynamics of egg phosphatidylcholine vesicles containing gangliosides. In: BBA - General Subjects. 1982 ; Vol. 647, No. 2. pp. 196-202.
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