Electrophoretic characterization of glutamic oxalacetic transaminase in human red cells of different ages

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Abstract

GOT isoenzymes of red cells from normal subjects, and from subjects with reticulocytosis have been analysed by cellogel electrophoresis. Hemolysates from normal and from relatively old red cells contain only the anodic fraction (GOT I); whereas hemolysates from subjects with reticulocytosis and from relatively young red cells contain, in addition, the cathodic fraction (GOT II). Since this isoenzyme is derived from mitochondria, it implies that these organelles are still present in reticulocytes and in young red cells.

Original languageEnglish
Pages (from-to)215-218
Number of pages4
JournalClinica Chimica Acta
Volume14
Issue number2
Publication statusPublished - Aug 1966

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Transaminases
Reticulocytosis
Cells
Isoenzymes
Mitochondria
Reticulocytes
Electrophoresis
Organelles

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

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abstract = "GOT isoenzymes of red cells from normal subjects, and from subjects with reticulocytosis have been analysed by cellogel electrophoresis. Hemolysates from normal and from relatively old red cells contain only the anodic fraction (GOT I); whereas hemolysates from subjects with reticulocytosis and from relatively young red cells contain, in addition, the cathodic fraction (GOT II). Since this isoenzyme is derived from mitochondria, it implies that these organelles are still present in reticulocytes and in young red cells.",
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N2 - GOT isoenzymes of red cells from normal subjects, and from subjects with reticulocytosis have been analysed by cellogel electrophoresis. Hemolysates from normal and from relatively old red cells contain only the anodic fraction (GOT I); whereas hemolysates from subjects with reticulocytosis and from relatively young red cells contain, in addition, the cathodic fraction (GOT II). Since this isoenzyme is derived from mitochondria, it implies that these organelles are still present in reticulocytes and in young red cells.

AB - GOT isoenzymes of red cells from normal subjects, and from subjects with reticulocytosis have been analysed by cellogel electrophoresis. Hemolysates from normal and from relatively old red cells contain only the anodic fraction (GOT I); whereas hemolysates from subjects with reticulocytosis and from relatively young red cells contain, in addition, the cathodic fraction (GOT II). Since this isoenzyme is derived from mitochondria, it implies that these organelles are still present in reticulocytes and in young red cells.

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