Electrophoretic pattern of NADPH-dependent oxidoreductive activities in K 562 and HL 60 leukemic cell lines

Roberto Ravazzolo, Renata Barresi, Cecilia Garré, Giovanna Bianchi-Scarrá, Franco Ajmar

Research output: Contribution to journalArticle

Abstract

Morphological and functional differentiation of hemopoietic cells is accompanied by the expression of lineage-specific protein markers. NADPH-oxidoreductive enzymatic activities in HL 60 and K 562 leukemic cell lines, compared with granulocytes and erythrocytes, show a NADPH-oxidizing and a NADPH-diaphorase activity. The oxidizing activity, absent in erythrocytes, has the same electrophoretic migration in HL 60 cells and granulocytes while it is different in K 562 cells. The diaphorase, absent in HL 60 cells and granulocytes, has the same migration in erythrocytes and K 562 cells, although with a slightly different quantitative expression. K 562 cells induced to differentiation with arabinofuranosylcytosine show the appearance of a band of NADPH-oxidizing activity of granulocytic type, together with the major band found in these cells.

Original languageEnglish
Pages (from-to)155-158
Number of pages4
JournalCell Differentiation
Volume22
Issue number2
DOIs
Publication statusPublished - 1988

Keywords

  • Hemopoietic differentiation
  • HL 60 cell line
  • K 562 cell line
  • NADPH-diaphorase
  • NADPH-oxidizing activity

ASJC Scopus subject areas

  • Developmental Biology

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