Electrophoretic separation of Aγ and Gγ human globin chains in nonidet P-40

Angelo Guerrasio, Giuseppe Saglio, Umberto Mazza, Piergiorgio Pich, Clara Camaschella, Giuseppe Ricco, Elisabetta Gianazza, Pier Giorgio Righetti

Research output: Contribution to journalArticle

Abstract

Electrophoresis in cellulose acetate in the presence of 3% Nonidet P-40 can resolve two neutral genetic variants, Aγ and Gγ human fetal globin chains. The ratio of these two chains, determined by densitometry of the electrophoretic strips, is in excellent agreement with the Gly-Ala ratio obtained by chemical analysis of the cyanogen bromide fragment 7CB3. It is suggested that the detergent binds preferentially to the hydrophobic amino acid segment 133-141 in the Aγ chain, thus masking either a Lys or an Arg residue at the two extremes.

Original languageEnglish
Pages (from-to)7-11
Number of pages5
JournalClinica Chimica Acta
Volume99
Issue number1
DOIs
Publication statusPublished - Nov 15 1979

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N-glycylalanine
Cellulose Acetate Electrophoresis
Cyanogen Bromide
Globins
Densitometry
Electrophoresis
Detergents
Amino Acids
Chemical analysis
Nonidet P-40
acetylcellulose

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Guerrasio, A., Saglio, G., Mazza, U., Pich, P., Camaschella, C., Ricco, G., ... Righetti, P. G. (1979). Electrophoretic separation of Aγ and Gγ human globin chains in nonidet P-40. Clinica Chimica Acta, 99(1), 7-11. https://doi.org/10.1016/0009-8981(79)90133-5

Electrophoretic separation of Aγ and Gγ human globin chains in nonidet P-40. / Guerrasio, Angelo; Saglio, Giuseppe; Mazza, Umberto; Pich, Piergiorgio; Camaschella, Clara; Ricco, Giuseppe; Gianazza, Elisabetta; Righetti, Pier Giorgio.

In: Clinica Chimica Acta, Vol. 99, No. 1, 15.11.1979, p. 7-11.

Research output: Contribution to journalArticle

Guerrasio, A, Saglio, G, Mazza, U, Pich, P, Camaschella, C, Ricco, G, Gianazza, E & Righetti, PG 1979, 'Electrophoretic separation of Aγ and Gγ human globin chains in nonidet P-40', Clinica Chimica Acta, vol. 99, no. 1, pp. 7-11. https://doi.org/10.1016/0009-8981(79)90133-5
Guerrasio A, Saglio G, Mazza U, Pich P, Camaschella C, Ricco G et al. Electrophoretic separation of Aγ and Gγ human globin chains in nonidet P-40. Clinica Chimica Acta. 1979 Nov 15;99(1):7-11. https://doi.org/10.1016/0009-8981(79)90133-5
Guerrasio, Angelo ; Saglio, Giuseppe ; Mazza, Umberto ; Pich, Piergiorgio ; Camaschella, Clara ; Ricco, Giuseppe ; Gianazza, Elisabetta ; Righetti, Pier Giorgio. / Electrophoretic separation of Aγ and Gγ human globin chains in nonidet P-40. In: Clinica Chimica Acta. 1979 ; Vol. 99, No. 1. pp. 7-11.
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