Electrophysiological properties of mouse bone marrow c-kit+ cells co-cultured onto neonatal cardiac myocytes

Laura Lagostena, Daniele Avitabile, Elena De Falco, Alessia Orlandi, Francesca Grassi, Maria Grazia Iachininoto, Gianluca Ragone, Sergio Fucile, Giulio Pompilio, Fabrizio Eusebi, Maurizio Pesce, Maurizio C. Capogrossi

Research output: Contribution to journalArticle

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Abstract

Objective: Controversy about hematopoietic stem cells reprogramming into cardiac myocytes is currently supported by positive and negative findings. In fact, some reports have shown the ability of stem cells from the bone marrow (BM) to differentiate into cardiac myocytes and to contribute to myocardium repair, while others have reported the opposite. Methods: C-kit+ cells from mouse bone marrow were co-cultured onto neonatal cardiac myocytes. Hematopoietic stem cell-derived cells were analyzed by investigating the expression of cardiac markers and ion channels and by single-cell electrophysiological recordings. Results: Groups of undifferentiated c-kit + cells displayed only outward currents. Co-cultured c-kit + stem cells on neonatal cardiac myocytes expressed cardiac markers and Na+ and Ca2+ voltage-gated ion channels. However, Na+ and Ca2+ currents were not detected by electrophysiological patch-clamp recordings even if caffeine and cyclopiazonic acid treatment showed the presence of intracellular calcium stores. This suggests that these channels, although expressed, were not functional and thus do not allow the coupling between excitation and contraction that is typical of cardiac myocytes. Nevertheless, co-cultured cells had a more hyperpolarized resting membrane potential and, at least in a subset of cells, displayed voltage-gated inward rectifier currents and outward currents. Co-cultured c-kit+-derived cells were not connected to surrounding cardiac myocytes through gap junctions. To induce a more pronounced differentiation, co-cultured cells were treated with BMP-4 and TGF-β, two factors that were shown to trigger a cardiac myocyte differentiation pathway in embryonic stem (ES) cells. Even under these conditions, c-kit+ cells did not differentiate into functionally active cardiac myocytes. However, TGF-β/BMP-4-treated cells were hyperpolarized and showed and increased inward rectifier current density. Conclusions: Our study shows that mouse BM hematopoietic stem cells exhibit a limited plasticity to transdifferentiate into cardiac myocytes in culture.

Original languageEnglish
Pages (from-to)482-492
Number of pages11
JournalCardiovascular Research
Volume66
Issue number3
DOIs
Publication statusPublished - Jun 1 2005

Fingerprint

Cardiac Myocytes
Cultured Cells
Bone Marrow
Hematopoietic Stem Cells
Ion Channels
Stem Cells
Excitation Contraction Coupling
Gap Junctions
Embryonic Stem Cells
Caffeine
Bone Marrow Cells
Membrane Potentials
Myocardium
Calcium

Keywords

  • BMP-4
  • Bone marrow
  • C-kit
  • Cardiac myocyte
  • Electrophysiology
  • Reprogramming
  • Stem cell
  • TGF-β

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Lagostena, L., Avitabile, D., De Falco, E., Orlandi, A., Grassi, F., Iachininoto, M. G., ... Capogrossi, M. C. (2005). Electrophysiological properties of mouse bone marrow c-kit+ cells co-cultured onto neonatal cardiac myocytes. Cardiovascular Research, 66(3), 482-492. https://doi.org/10.1016/j.cardiores.2005.01.018

Electrophysiological properties of mouse bone marrow c-kit+ cells co-cultured onto neonatal cardiac myocytes. / Lagostena, Laura; Avitabile, Daniele; De Falco, Elena; Orlandi, Alessia; Grassi, Francesca; Iachininoto, Maria Grazia; Ragone, Gianluca; Fucile, Sergio; Pompilio, Giulio; Eusebi, Fabrizio; Pesce, Maurizio; Capogrossi, Maurizio C.

In: Cardiovascular Research, Vol. 66, No. 3, 01.06.2005, p. 482-492.

Research output: Contribution to journalArticle

Lagostena, L, Avitabile, D, De Falco, E, Orlandi, A, Grassi, F, Iachininoto, MG, Ragone, G, Fucile, S, Pompilio, G, Eusebi, F, Pesce, M & Capogrossi, MC 2005, 'Electrophysiological properties of mouse bone marrow c-kit+ cells co-cultured onto neonatal cardiac myocytes', Cardiovascular Research, vol. 66, no. 3, pp. 482-492. https://doi.org/10.1016/j.cardiores.2005.01.018
Lagostena, Laura ; Avitabile, Daniele ; De Falco, Elena ; Orlandi, Alessia ; Grassi, Francesca ; Iachininoto, Maria Grazia ; Ragone, Gianluca ; Fucile, Sergio ; Pompilio, Giulio ; Eusebi, Fabrizio ; Pesce, Maurizio ; Capogrossi, Maurizio C. / Electrophysiological properties of mouse bone marrow c-kit+ cells co-cultured onto neonatal cardiac myocytes. In: Cardiovascular Research. 2005 ; Vol. 66, No. 3. pp. 482-492.
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AU - Orlandi, Alessia

AU - Grassi, Francesca

AU - Iachininoto, Maria Grazia

AU - Ragone, Gianluca

AU - Fucile, Sergio

AU - Pompilio, Giulio

AU - Eusebi, Fabrizio

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N2 - Objective: Controversy about hematopoietic stem cells reprogramming into cardiac myocytes is currently supported by positive and negative findings. In fact, some reports have shown the ability of stem cells from the bone marrow (BM) to differentiate into cardiac myocytes and to contribute to myocardium repair, while others have reported the opposite. Methods: C-kit+ cells from mouse bone marrow were co-cultured onto neonatal cardiac myocytes. Hematopoietic stem cell-derived cells were analyzed by investigating the expression of cardiac markers and ion channels and by single-cell electrophysiological recordings. Results: Groups of undifferentiated c-kit + cells displayed only outward currents. Co-cultured c-kit + stem cells on neonatal cardiac myocytes expressed cardiac markers and Na+ and Ca2+ voltage-gated ion channels. However, Na+ and Ca2+ currents were not detected by electrophysiological patch-clamp recordings even if caffeine and cyclopiazonic acid treatment showed the presence of intracellular calcium stores. This suggests that these channels, although expressed, were not functional and thus do not allow the coupling between excitation and contraction that is typical of cardiac myocytes. Nevertheless, co-cultured cells had a more hyperpolarized resting membrane potential and, at least in a subset of cells, displayed voltage-gated inward rectifier currents and outward currents. Co-cultured c-kit+-derived cells were not connected to surrounding cardiac myocytes through gap junctions. To induce a more pronounced differentiation, co-cultured cells were treated with BMP-4 and TGF-β, two factors that were shown to trigger a cardiac myocyte differentiation pathway in embryonic stem (ES) cells. Even under these conditions, c-kit+ cells did not differentiate into functionally active cardiac myocytes. However, TGF-β/BMP-4-treated cells were hyperpolarized and showed and increased inward rectifier current density. Conclusions: Our study shows that mouse BM hematopoietic stem cells exhibit a limited plasticity to transdifferentiate into cardiac myocytes in culture.

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