Electrophysiological study of Single gold nanoparticle/α-Hemolysin complex formation: A nanotool to slow down ssDNA through the α-Hemolysin nanopore

Yann Astier, Oktay Uzun, Francesco Stellacci

Research output: Contribution to journalArticle

Abstract

A method to trap single-monolayer-protected gold nanoparticles in the α-hemolysin (αHL) nanopore was reported. The method also demonstrated conductance signature of a single sodium 3-mercapto-1- propanesulfonate (MPSA)-coated gold nanocrystal trapped inside the αHL. The trans-membrane potential amplitude impact on the size of the nanoparticle inside the pore and correlation between the size of trapped nanoparticle and its dwell time were also reported. It was observed that the largest nanoparticle entered in the pore yielded a 50% block of the pore conductance. Gold nanoparticles coated with MPSA and mixtures of MPSA/octanethiol (OT) organic ligands were synthesized for the study. It was also found that the particles with small diameter were able to individually enter in αHL nanopore cavity when a potential was applied to accelerate the negatively charges nanoparticles.

Original languageEnglish
Pages (from-to)1273-1278
Number of pages6
JournalSmall
Volume5
Issue number11
DOIs
Publication statusPublished - Jun 5 2009

Keywords

  • α-Hemolysin
  • Gold nanoparticles
  • Nanopores
  • Proteins
  • Single-particle studies

ASJC Scopus subject areas

  • Biomaterials
  • Engineering (miscellaneous)
  • Biotechnology
  • Medicine(all)

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