Electroporated DNA Vaccine Clears Away Multifocal Mammary Carcinomas in Her-2/neu Transgenic Mice

Elena Quaglino, Manuela Iezzi, Cristina Mastini, Augusto Amici, Federica Pericle, Emma Di Carlo, Serenella M. Pupa, Carla De Giovanni, Michela Spadaro, Claudia Curcio, Pier Luigi Lollini, Piero Musiani, Guido Forni, Federica Cavallo

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Abstract

The transforming rat Her-2/neu oncogene embedded into the genome of virgin transgenic BALB/c mice (BALB-neuT) provokes the development of an invasive carcinoma in each of their 10 mammary glands. i.m. vaccination with DNA plasmids coding for the extracellular and transmembrane domains of the protein product of the Her-2/neu oncogene started when mice already display multifocal in situ carcinomas temporarily halts neoplastic progression, but all mice develop a tumor by week 43. By contrast, progressive clearance of neoplastic lesions and complete protection of all 1-year-old mice are achieved when the same plasmids are electroporated at 10-week intervals. Pathological findings, in vitro tests, and the results from the immunization of both IFN-γ and immunoglobulin gene knockout BALB-neuT mice, and of adoptive transfer experiments, all suggest that tumor clearance rests on the combination of antibodies and IFN-γ-releasing T cells. These findings show that an appropriate vaccine effectively inhibits the progression of multifocal preneoplastic lesions.

Original languageEnglish
Pages (from-to)2858-2864
Number of pages7
JournalCancer Research
Volume64
Issue number8
DOIs
Publication statusPublished - Apr 15 2004

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Quaglino, E., Iezzi, M., Mastini, C., Amici, A., Pericle, F., Di Carlo, E., Pupa, S. M., De Giovanni, C., Spadaro, M., Curcio, C., Lollini, P. L., Musiani, P., Forni, G., & Cavallo, F. (2004). Electroporated DNA Vaccine Clears Away Multifocal Mammary Carcinomas in Her-2/neu Transgenic Mice. Cancer Research, 64(8), 2858-2864. https://doi.org/10.1158/0008-5472.CAN-03-2962