Abstract
The transforming rat Her-2/neu oncogene embedded into the genome of virgin transgenic BALB/c mice (BALB-neuT) provokes the development of an invasive carcinoma in each of their 10 mammary glands. i.m. vaccination with DNA plasmids coding for the extracellular and transmembrane domains of the protein product of the Her-2/neu oncogene started when mice already display multifocal in situ carcinomas temporarily halts neoplastic progression, but all mice develop a tumor by week 43. By contrast, progressive clearance of neoplastic lesions and complete protection of all 1-year-old mice are achieved when the same plasmids are electroporated at 10-week intervals. Pathological findings, in vitro tests, and the results from the immunization of both IFN-γ and immunoglobulin gene knockout BALB-neuT mice, and of adoptive transfer experiments, all suggest that tumor clearance rests on the combination of antibodies and IFN-γ-releasing T cells. These findings show that an appropriate vaccine effectively inhibits the progression of multifocal preneoplastic lesions.
| Original language | English |
|---|---|
| Pages (from-to) | 2858-2864 |
| Number of pages | 7 |
| Journal | Cancer Research |
| Volume | 64 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Apr 15 2004 |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Electroporated DNA Vaccine Clears Away Multifocal Mammary Carcinomas in Her-2/neu Transgenic Mice. / Quaglino, Elena; Iezzi, Manuela; Mastini, Cristina; Amici, Augusto; Pericle, Federica; Di Carlo, Emma; Pupa, Serenella M.; De Giovanni, Carla; Spadaro, Michela; Curcio, Claudia; Lollini, Pier Luigi; Musiani, Piero; Forni, Guido; Cavallo, Federica.
In: Cancer Research, Vol. 64, No. 8, 15.04.2004, p. 2858-2864.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Electroporated DNA Vaccine Clears Away Multifocal Mammary Carcinomas in Her-2/neu Transgenic Mice
AU - Quaglino, Elena
AU - Iezzi, Manuela
AU - Mastini, Cristina
AU - Amici, Augusto
AU - Pericle, Federica
AU - Di Carlo, Emma
AU - Pupa, Serenella M.
AU - De Giovanni, Carla
AU - Spadaro, Michela
AU - Curcio, Claudia
AU - Lollini, Pier Luigi
AU - Musiani, Piero
AU - Forni, Guido
AU - Cavallo, Federica
PY - 2004/4/15
Y1 - 2004/4/15
N2 - The transforming rat Her-2/neu oncogene embedded into the genome of virgin transgenic BALB/c mice (BALB-neuT) provokes the development of an invasive carcinoma in each of their 10 mammary glands. i.m. vaccination with DNA plasmids coding for the extracellular and transmembrane domains of the protein product of the Her-2/neu oncogene started when mice already display multifocal in situ carcinomas temporarily halts neoplastic progression, but all mice develop a tumor by week 43. By contrast, progressive clearance of neoplastic lesions and complete protection of all 1-year-old mice are achieved when the same plasmids are electroporated at 10-week intervals. Pathological findings, in vitro tests, and the results from the immunization of both IFN-γ and immunoglobulin gene knockout BALB-neuT mice, and of adoptive transfer experiments, all suggest that tumor clearance rests on the combination of antibodies and IFN-γ-releasing T cells. These findings show that an appropriate vaccine effectively inhibits the progression of multifocal preneoplastic lesions.
AB - The transforming rat Her-2/neu oncogene embedded into the genome of virgin transgenic BALB/c mice (BALB-neuT) provokes the development of an invasive carcinoma in each of their 10 mammary glands. i.m. vaccination with DNA plasmids coding for the extracellular and transmembrane domains of the protein product of the Her-2/neu oncogene started when mice already display multifocal in situ carcinomas temporarily halts neoplastic progression, but all mice develop a tumor by week 43. By contrast, progressive clearance of neoplastic lesions and complete protection of all 1-year-old mice are achieved when the same plasmids are electroporated at 10-week intervals. Pathological findings, in vitro tests, and the results from the immunization of both IFN-γ and immunoglobulin gene knockout BALB-neuT mice, and of adoptive transfer experiments, all suggest that tumor clearance rests on the combination of antibodies and IFN-γ-releasing T cells. These findings show that an appropriate vaccine effectively inhibits the progression of multifocal preneoplastic lesions.
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UR - http://www.scopus.com/inward/citedby.url?scp=4043147286&partnerID=8YFLogxK
U2 - 10.1158/0008-5472.CAN-03-2962
DO - 10.1158/0008-5472.CAN-03-2962
M3 - Article
C2 - 15087404
AN - SCOPUS:4043147286
VL - 64
SP - 2858
EP - 2864
JO - Journal of Cancer Research
JF - Journal of Cancer Research
SN - 0008-5472
IS - 8
ER -