Electroporation adopting trains of biphasic pulses enhances in vitro and in vivo the cytotoxic effect of doxorubicin on multidrug resistant colon adenocarcinoma cells (LoVo)

Stefania Meschini, Maria Condello, Pasquale Lista, Bruno Vincenzi, Alfonso Baldi, Gennaro Citro, Giuseppe Arancia, Enrico P. Spugnini

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Few articles in the literature have focused on electroporation as a strategy to reverse multidrug resistance (MDR) of tumour cells and they are mostly limited to the improved efficacy of bleomycin. We tested the application of trains of biphasic pulses to cell suspensions and to murine xenografts as a strategy to increase the uptake of doxorubicin (DOX) and to enhance its cytotoxicity against chemoresistant cells. The human colon adenocarcinoma cell line LoVo DX, expressing MDR phenotype with high levels of P-glycoprotein (P-gp), has been used. The in vitro and in vivo studies gave the following results: (i) the application of the electric pulses to the cell suspension, immediately before DOX administration, induced a significant increase of drug retention; (ii) confocal microscopy observations showed a remarkable increase of intranuclear accumulation of DOX induced by electroporation; (iii) cell survival assay revealed a decrease of cell viability in the cultures treated with the combination of electroporation and doxorubicin; (iv) scanning electron microscopy observations revealed consistent morphological changes after the combined exposure to electroporation and doxorubicin; (v) in implanted mice the combined treatment induced an evident slowdown on the tumour growth when compared to treatment with DOX alone; (vi) histopathological analysis evidenced tumour destruction and its replacement by scar tissue in the tumours treated with the combination of doxorubicin and electroporation.

Original languageEnglish
Pages (from-to)2236-2243
Number of pages8
JournalEuropean Journal of Cancer
Volume48
Issue number14
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Electroporation
Doxorubicin
Colon
Adenocarcinoma
Multiple Drug Resistance
Neoplasms
Cell Survival
Suspensions
Bleomycin
P-Glycoprotein
In Vitro Techniques
Heterografts
Confocal Microscopy
Electron Scanning Microscopy
Cicatrix
Phenotype
Cell Line
Growth
Pharmaceutical Preparations

Keywords

  • Colon carcinoma cells
  • Doxorubicin
  • Electroporation
  • Multidrug resistance

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Electroporation adopting trains of biphasic pulses enhances in vitro and in vivo the cytotoxic effect of doxorubicin on multidrug resistant colon adenocarcinoma cells (LoVo). / Meschini, Stefania; Condello, Maria; Lista, Pasquale; Vincenzi, Bruno; Baldi, Alfonso; Citro, Gennaro; Arancia, Giuseppe; Spugnini, Enrico P.

In: European Journal of Cancer, Vol. 48, No. 14, 09.2012, p. 2236-2243.

Research output: Contribution to journalArticle

Meschini, Stefania ; Condello, Maria ; Lista, Pasquale ; Vincenzi, Bruno ; Baldi, Alfonso ; Citro, Gennaro ; Arancia, Giuseppe ; Spugnini, Enrico P. / Electroporation adopting trains of biphasic pulses enhances in vitro and in vivo the cytotoxic effect of doxorubicin on multidrug resistant colon adenocarcinoma cells (LoVo). In: European Journal of Cancer. 2012 ; Vol. 48, No. 14. pp. 2236-2243.
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