Electroporation of skeletal muscle induces danger signal release and antigen-presenting cell recruitment independently of DNA vaccine administration

Pieranna Chiarella, Emanuela Massi, Mariangela De Robertis, Annarita Sibilio, Paola Parrella, Vito Michele Fazio, Emanuela Signori

Research output: Contribution to journalArticle

Abstract

Background: Plasmid DNA vaccination combined with electroporation (EP) provides a promising approach for the prevention of infectious diseases and for cancer immunotherapy. This technology has been described as being effective in activating humoural and cellular immune response in the host as well as in enhancing expression of the encoded antigen. Several reports showed EP has adjuvant-like properties when combined with plasmid DNA injection although the effect in the absence of DNA has not been investigated. Objective: The aim of this study is to clarify whether the application of EP alone to the skeletal muscle is able to recruit and trigger cells involved in antigen presentation and immune response. Methods: Mouse skeletal muscle treated by EP were observed and processed for clinical, histological and immunohistochemistry analysis at different time points. Results: We demonstrate that EP induces transient morphological changes in the muscle with early production of endogenous cytokines responsible for signalling danger at the local level. Moreover, it causes the recruitment of inflammatory cells independently of the DNA injection and the activation of a danger pro-inflammatory pathway, resulting in T-lymphocyte migration. Conclusions: Our data indicate EP by itself is able to recruit and trigger cells involved in antigen presentation and immune response; hence, the idea that EP has adjuvant-like properties owing to a moderate tissue injury and generation of a pro-inflammatory contex-t with cytokine release that enhances the immune response. We suggest EP may be of practical use in clinical protocols, contributing to the development of DNA vaccination strategies.

Original languageEnglish
Pages (from-to)1645-1657
Number of pages13
JournalExpert Opinion on Biological Therapy
Volume8
Issue number11
DOIs
Publication statusPublished - Nov 2008

Fingerprint

DNA Vaccines
Electroporation
Antigen-Presenting Cells
Muscle
Skeletal Muscle
DNA
Histocompatibility Antigens Class II
Plasmids
Cytokines
Antigen Presentation
T-cells
Vaccination
Injections
Chemical activation
Tissue
Antigens
Clinical Protocols
Cellular Immunity
Immunotherapy
Communicable Diseases

Keywords

  • Adjuvant
  • Antigen presentation
  • Dendritic cell
  • DNA vaccine
  • Electroporation

ASJC Scopus subject areas

  • Pharmacology
  • Clinical Biochemistry
  • Drug Discovery

Cite this

Electroporation of skeletal muscle induces danger signal release and antigen-presenting cell recruitment independently of DNA vaccine administration. / Chiarella, Pieranna; Massi, Emanuela; De Robertis, Mariangela; Sibilio, Annarita; Parrella, Paola; Fazio, Vito Michele; Signori, Emanuela.

In: Expert Opinion on Biological Therapy, Vol. 8, No. 11, 11.2008, p. 1645-1657.

Research output: Contribution to journalArticle

Chiarella, Pieranna ; Massi, Emanuela ; De Robertis, Mariangela ; Sibilio, Annarita ; Parrella, Paola ; Fazio, Vito Michele ; Signori, Emanuela. / Electroporation of skeletal muscle induces danger signal release and antigen-presenting cell recruitment independently of DNA vaccine administration. In: Expert Opinion on Biological Therapy. 2008 ; Vol. 8, No. 11. pp. 1645-1657.
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