Abstract
Allergic rhinitis (AR) is characterized by a Th2 polarized immune response, and specific immunotherapy modifies this arrangement, restoring a physiologic Th1 profile. Sublingual immunotherapy (SLIT) is widely prescribed. The aim of the study is to evaluate two different methods for assessing IFN-γ, namely ELISPOT and ELISA, before and after a pre-seasonal SLIT course as marker for Th1 response. Thirty-eight AR patients with pollen allergy assumed pre-seasonal SLIT for 3 months. Patients' blood samples for assessing IFN-γ serum levels were collected before initiating SLIT (baseline - T0), after 3 months pre-seasonal SLIT course (T1), and three months after completion of SLIT (T2). IFN-γ-specific producing cells, after allergen stimulation, were assessed by cytokine ELISPOT at the same time points. IFN-γ-specific producing cells significantly increased after SLIT both at T1 and T2 (p=0.0002). On the contrary, ELISA assessment did not reveal an increase in IFN-γ serum levels at any time point. In conclusion, these results demonstrate that ELISA assessment of serum IFN-γ is not suitable for identifying an early response.
| Original language | English |
|---|---|
| Pages (from-to) | 31-35 |
| Number of pages | 5 |
| Journal | European Journal of Inflammation |
| Volume | 8 |
| Issue number | 1 |
| Publication status | Published - Jan 2010 |
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Keywords
- Allergen
- Allergic rhinitis
- ELISA
- ELISPOT
- IFN-γ
- Pollens
- Sublingual immunotherapy
- Th1 cells
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
ELISPOT and ELISA assessment of interferon-gamma after sublingual immunotherapy. / Ciprandi, Giorgio; Fenoglio, D.; Ferrera, F.; De Amici, M.; Marseglia, G.
In: European Journal of Inflammation, Vol. 8, No. 1, 01.2010, p. 31-35.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - ELISPOT and ELISA assessment of interferon-gamma after sublingual immunotherapy
AU - Ciprandi, Giorgio
AU - Fenoglio, D.
AU - Ferrera, F.
AU - De Amici, M.
AU - Marseglia, G.
PY - 2010/1
Y1 - 2010/1
N2 - Allergic rhinitis (AR) is characterized by a Th2 polarized immune response, and specific immunotherapy modifies this arrangement, restoring a physiologic Th1 profile. Sublingual immunotherapy (SLIT) is widely prescribed. The aim of the study is to evaluate two different methods for assessing IFN-γ, namely ELISPOT and ELISA, before and after a pre-seasonal SLIT course as marker for Th1 response. Thirty-eight AR patients with pollen allergy assumed pre-seasonal SLIT for 3 months. Patients' blood samples for assessing IFN-γ serum levels were collected before initiating SLIT (baseline - T0), after 3 months pre-seasonal SLIT course (T1), and three months after completion of SLIT (T2). IFN-γ-specific producing cells, after allergen stimulation, were assessed by cytokine ELISPOT at the same time points. IFN-γ-specific producing cells significantly increased after SLIT both at T1 and T2 (p=0.0002). On the contrary, ELISA assessment did not reveal an increase in IFN-γ serum levels at any time point. In conclusion, these results demonstrate that ELISA assessment of serum IFN-γ is not suitable for identifying an early response.
AB - Allergic rhinitis (AR) is characterized by a Th2 polarized immune response, and specific immunotherapy modifies this arrangement, restoring a physiologic Th1 profile. Sublingual immunotherapy (SLIT) is widely prescribed. The aim of the study is to evaluate two different methods for assessing IFN-γ, namely ELISPOT and ELISA, before and after a pre-seasonal SLIT course as marker for Th1 response. Thirty-eight AR patients with pollen allergy assumed pre-seasonal SLIT for 3 months. Patients' blood samples for assessing IFN-γ serum levels were collected before initiating SLIT (baseline - T0), after 3 months pre-seasonal SLIT course (T1), and three months after completion of SLIT (T2). IFN-γ-specific producing cells, after allergen stimulation, were assessed by cytokine ELISPOT at the same time points. IFN-γ-specific producing cells significantly increased after SLIT both at T1 and T2 (p=0.0002). On the contrary, ELISA assessment did not reveal an increase in IFN-γ serum levels at any time point. In conclusion, these results demonstrate that ELISA assessment of serum IFN-γ is not suitable for identifying an early response.
KW - Allergen
KW - Allergic rhinitis
KW - ELISA
KW - ELISPOT
KW - IFN-γ
KW - Pollens
KW - Sublingual immunotherapy
KW - Th1 cells
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UR - http://www.scopus.com/inward/citedby.url?scp=77952710866&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:77952710866
VL - 8
SP - 31
EP - 35
JO - European Journal of Inflammation
JF - European Journal of Inflammation
SN - 1721-727X
IS - 1
ER -