Elucidation of signal transduction pathways by transfection of cells with modified oncogenes.

Linda S. Steelman, William L. Blalock, Xiao Yang Wang, Phillip W. Moye, John T. Lee, John G. Shelton, Patrick M. Navolanic, Julianne M. Davis, Steven L. Knapp, Richard A. Franklin, Martyn K. White, James A. McCubrey

Research output: Contribution to journalArticle

Abstract

This chapter will focus on introduction of various wild type (WT) and mutant genes into cells by DNA transfection. Techniques for analysis of the inheritance, expression, and biological effects of the introduced genes will be described. Various strong and weak points about three different techniques of stable gene transfer, including calcium-phosphate DNA precipitation, transfection via liposomes, and transfection via electroporation, will be discussed.

Original languageEnglish
Pages (from-to)203-220
Number of pages18
JournalMethods in molecular biology (Clifton, N.J.)
Volume218
Publication statusPublished - 2003

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Oncogenes
Transfection
Signal Transduction
Gene Transfer Techniques
Electroporation
DNA
Liposomes
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Steelman, L. S., Blalock, W. L., Wang, X. Y., Moye, P. W., Lee, J. T., Shelton, J. G., ... McCubrey, J. A. (2003). Elucidation of signal transduction pathways by transfection of cells with modified oncogenes. Methods in molecular biology (Clifton, N.J.), 218, 203-220.

Elucidation of signal transduction pathways by transfection of cells with modified oncogenes. / Steelman, Linda S.; Blalock, William L.; Wang, Xiao Yang; Moye, Phillip W.; Lee, John T.; Shelton, John G.; Navolanic, Patrick M.; Davis, Julianne M.; Knapp, Steven L.; Franklin, Richard A.; White, Martyn K.; McCubrey, James A.

In: Methods in molecular biology (Clifton, N.J.), Vol. 218, 2003, p. 203-220.

Research output: Contribution to journalArticle

Steelman, LS, Blalock, WL, Wang, XY, Moye, PW, Lee, JT, Shelton, JG, Navolanic, PM, Davis, JM, Knapp, SL, Franklin, RA, White, MK & McCubrey, JA 2003, 'Elucidation of signal transduction pathways by transfection of cells with modified oncogenes.', Methods in molecular biology (Clifton, N.J.), vol. 218, pp. 203-220.
Steelman, Linda S. ; Blalock, William L. ; Wang, Xiao Yang ; Moye, Phillip W. ; Lee, John T. ; Shelton, John G. ; Navolanic, Patrick M. ; Davis, Julianne M. ; Knapp, Steven L. ; Franklin, Richard A. ; White, Martyn K. ; McCubrey, James A. / Elucidation of signal transduction pathways by transfection of cells with modified oncogenes. In: Methods in molecular biology (Clifton, N.J.). 2003 ; Vol. 218. pp. 203-220.
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