Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene

Pedro Luis Herrera, Joaquin Huarte, Francesca Sanvito, Paolo Meda, Lelio Orci, Jean Dominique Vassalli

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

By immunofluorescence on cytospin preparations and on semithin sections of mouse pancreatic buds, we have found glucagon and pancreatic polypeptide (PP)-containing cells at embryonal day 10.5 (E 10.5) in dorsal buds and at E 11.5 in ventral buds. Insulin-containing cells appear in dorsal buds at E 11.5, and one to two days later in ventral buds. Somatostatin-containing cells are detectable from E 13.5 in both dorsal and ventral buds. A quantitative analysis shows that up to E 15.5, PP-containing cells are relatively abundant in both buds. By PCR amplification of oligo(dT)-primed cDNAs prepared from total pancreatic RNA, we also detect PP mRNA from E 10.5 onwards, thus confirming the early expression of the PP gene in the developing mouse pancreas. Analysis of endocrine cells in situ suggests three major patterns of cell distribution in embryonic pancreas. First, individual hormone-containing cells are located within the epithelium of pancreatic ducts. In both dorsal and ventral buds, the majority of these endocrine cells contain PP, but many also contain glucagon, insulin or somatostatin. Secondly, clusters of endocrine cells are found in the pancreatic interstitium. Many of these cells contain both glucagon and PP which, by immunogold labelling of consecutive thin sections, can be shown to co-exist within individual secretory granules. Finally, starting on E 18.5, typical islets are formed with centrally located B cells and with the adult 'one cell - one hormone' phenotype. These results suggest an intriguing ontogenic relationship between A- and PP-cells, and also indicate that PP-containing cells may occupy a hitherto unexpected place in the lineage of endocrine islet cells.

Original languageEnglish
Pages (from-to)1257-1265
Number of pages9
JournalDevelopment
Volume113
Issue number4
Publication statusPublished - Dec 1991

Fingerprint

Pancreatic Polypeptide
Pancreatic Polypeptide-Secreting Cells
Islets of Langerhans
Endocrine Cells
Embryonic Development
Glucagon
Genes
Pancreas
Hormones
Insulin
Somatostatin-Secreting Cells
Pancreatic Ducts
Secretory Vesicles
Somatostatin
Fluorescent Antibody Technique
B-Lymphocytes
Epithelium
Complementary DNA
RNA
Phenotype

Keywords

  • Embryo
  • Islet
  • Mouse
  • Pancreas
  • Pancreatic polypeptide

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Herrera, P. L., Huarte, J., Sanvito, F., Meda, P., Orci, L., & Vassalli, J. D. (1991). Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene. Development, 113(4), 1257-1265.

Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene. / Herrera, Pedro Luis; Huarte, Joaquin; Sanvito, Francesca; Meda, Paolo; Orci, Lelio; Vassalli, Jean Dominique.

In: Development, Vol. 113, No. 4, 12.1991, p. 1257-1265.

Research output: Contribution to journalArticle

Herrera, PL, Huarte, J, Sanvito, F, Meda, P, Orci, L & Vassalli, JD 1991, 'Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene', Development, vol. 113, no. 4, pp. 1257-1265.
Herrera PL, Huarte J, Sanvito F, Meda P, Orci L, Vassalli JD. Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene. Development. 1991 Dec;113(4):1257-1265.
Herrera, Pedro Luis ; Huarte, Joaquin ; Sanvito, Francesca ; Meda, Paolo ; Orci, Lelio ; Vassalli, Jean Dominique. / Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene. In: Development. 1991 ; Vol. 113, No. 4. pp. 1257-1265.
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