Embryonic stem and haematopoietic progenitor cells resist to Aβ oligomer toxicity and maintain the differentiation potency in culture

Tui Neri, Monica Bucciantini, Vittorio Rosti, Sara Raimondi, Annalisa Relini, Margherita Massa, Maurizio Zuccotti, Simona Donadei, Massimo Stefani, Carlo Alberto Redi, Giampaolo Merlini, Monica Stoppini, Silvia Garagna, Vittorio Bellotti

Research output: Contribution to journalArticlepeer-review

Abstract

Regenerative medicine deals with the possible use of stem cells to repair tissues damaged by aging and related diseases, including amyloidoses. In the latter case, the toxicity of the amyloid deposits can, in principle, question the possibility to graft specific tissues by undifferentiated cells. To assess whether stem cells are vulnerable to amyloid toxicity, we exposed, in culture, murine embryonic stem ES cells and haematopoietic progenitor HP cells to oligomers of the amyloidogenic peptide Aβ42 at concentrations previously shown to be cytotoxic to several other cell types. These stem cells did not display any sign of apoptosis and their survival, proliferation and differentiation were not affected by the oligomers although the MTT 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide assay revealed that ES, but not HP, cells displayed some impaired ability to reduce the tetrazole salts possibly as a result of transient oxidative stress. Our results support a remarkable resistance of the investigated stem cells against amyloids and hence their potential use in cell therapy of Alzheimer's disease and, possibly, other amyloid diseases.

Original languageEnglish
Pages (from-to)137-145
Number of pages9
JournalAmyloid
Volume17
Issue number3-4
DOIs
Publication statusPublished - Sep 2010

Keywords

  • Amyloid oligomer toxicity
  • cell therapy
  • stem cells

ASJC Scopus subject areas

  • Internal Medicine

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