Emended description of mycobacterium abscessus mycobacterium abscessus subsp. Abscessus and mycobacterium abscessus subsp. bolletii and designation of mycobacterium abscessus subsp. massiliense comb. nov.

Enrico Tortoli, Thomas A. Kohl, Barbara A. Brown-Elliott, Alberto Trovato, Sylvia Cardoso Leão, Maria Jesus Garcia, Sruthi Vasireddy, Christine Y. Turenne, David E. Griffith, Julie V. Philley, Rossella Baldan, Silvia Campana, Lisa Cariani, Carla Colombo, Giovanni Taccetti, Antonio Teri, Stefan Niemann, Richard J. Wallace, Daniela M. Cirillo

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The taxonomic position of members of the Mycobacterium abscessus complex has been the subject of intensive investigation and, in some aspects confusion, in recent years as a result of varying approaches to genetic data interpretation. Currently, the former species Mycobacterium massiliense and Mycobacterium bolletii are grouped together as Mycobacterium abscessus subsp. bolletii. They differ greatly, however, as the former M. bolletii has a functional erm(41) gene that confers inducible resistance to macrolides, the primary therapeutic antimicrobials for M. abscessus, while in the former M. massiliense the erm(41) gene is non-functional. Furthermore, previous whole genome studies of the M. abscessus group support the separation of M. bolletii and M. massiliense. To shed further light on the population structure of Mycobacterium abscessus, 43 strains and three genomes retrieved from GenBank were subjected to pairwise comparisons using three computational approaches: verage ucleotide dentity, enome to enome istance and single nucleotide polymorphism analysis. The three methods produced overlapping results, each demonstrating three clusters of strains corresponding to the same number of taxonomic entities. The distances were insufficient to warrant distinction at the species level, but met the criteria for differentiation at the subspecies level. Based on prior erm(41)-related phenotypic data and current genomic data, we conclude that the species M. abscessus encompasses, in adjunct to the presently recognized subspecies M. abscessus subsp. abscessus and M. abscessus subsp. bolletii, a third subspecies for which we suggest the name M. abscessus subsp. massiliense comb. nov. (type strain CCUG 48898T=CIP 108297T=DSM 45103T=KCTC 19086T).

Original languageEnglish
Article number001376
Pages (from-to)4471-4479
Number of pages9
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume66
Issue number11
DOIs
Publication statusPublished - Nov 1 2016

Fingerprint

aprepitant
Mycobacterium abscessus
Comb and Wattles
Mycobacterium
subspecies
genome
gene
data interpretation
population structure
Genome
genomics
polymorphism
Nucleic Acid Databases
Macrolides
Genes
Names
Single Nucleotide Polymorphism
macrolides

Keywords

  • Mycobacterium abscessus
  • Mycobacterium bolletii
  • Mycobacterium massiliense
  • Subspecies
  • Whole Genome Sequencing

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Emended description of mycobacterium abscessus mycobacterium abscessus subsp. Abscessus and mycobacterium abscessus subsp. bolletii and designation of mycobacterium abscessus subsp. massiliense comb. nov. / Tortoli, Enrico; Kohl, Thomas A.; Brown-Elliott, Barbara A.; Trovato, Alberto; Leão, Sylvia Cardoso; Garcia, Maria Jesus; Vasireddy, Sruthi; Turenne, Christine Y.; Griffith, David E.; Philley, Julie V.; Baldan, Rossella; Campana, Silvia; Cariani, Lisa; Colombo, Carla; Taccetti, Giovanni; Teri, Antonio; Niemann, Stefan; Wallace, Richard J.; Cirillo, Daniela M.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 66, No. 11, 001376, 01.11.2016, p. 4471-4479.

Research output: Contribution to journalArticle

Tortoli, E, Kohl, TA, Brown-Elliott, BA, Trovato, A, Leão, SC, Garcia, MJ, Vasireddy, S, Turenne, CY, Griffith, DE, Philley, JV, Baldan, R, Campana, S, Cariani, L, Colombo, C, Taccetti, G, Teri, A, Niemann, S, Wallace, RJ & Cirillo, DM 2016, 'Emended description of mycobacterium abscessus mycobacterium abscessus subsp. Abscessus and mycobacterium abscessus subsp. bolletii and designation of mycobacterium abscessus subsp. massiliense comb. nov.', International Journal of Systematic and Evolutionary Microbiology, vol. 66, no. 11, 001376, pp. 4471-4479. https://doi.org/10.1099/ijsem.0.001376
Tortoli, Enrico ; Kohl, Thomas A. ; Brown-Elliott, Barbara A. ; Trovato, Alberto ; Leão, Sylvia Cardoso ; Garcia, Maria Jesus ; Vasireddy, Sruthi ; Turenne, Christine Y. ; Griffith, David E. ; Philley, Julie V. ; Baldan, Rossella ; Campana, Silvia ; Cariani, Lisa ; Colombo, Carla ; Taccetti, Giovanni ; Teri, Antonio ; Niemann, Stefan ; Wallace, Richard J. ; Cirillo, Daniela M. / Emended description of mycobacterium abscessus mycobacterium abscessus subsp. Abscessus and mycobacterium abscessus subsp. bolletii and designation of mycobacterium abscessus subsp. massiliense comb. nov. In: International Journal of Systematic and Evolutionary Microbiology. 2016 ; Vol. 66, No. 11. pp. 4471-4479.
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