Emergence of a VIM-1 MBL and CTX-M-15 ESβL-producing Klebsiella pneumoniae clone from acute and rehabilitation hospitals in Italy

Elisabetta Nucleo, Melissa Spalla, Aurora Piazza, Maria Sofia Caltagirone, Sara Asticcioli, Maurizia Debiaggi, Carolina Matti, Rossana Daturi, Antonella Navarra, Maria Labonia, Roberta Migliavacca

Research output: Contribution to journalArticle

Abstract

We report the emergence of VIM-1 MBL and CTX-M-15-producing K. pneumoniae isolates collected from patients at two acute care hospitals (I.R.C.C.S. "S. Matteo" and "Casa Sollievo della Sofferenza" Hospital) and a long-term rehabilitation facility in Northern Italy (I.R.C.C.S. "S. Maugeri"). Between February 2007 and October 2008, 30 K. pneumoniae strains showing decreased susceptibility to carbapenems were collected. PCR and sequencing experiments revealed the presence of blaVIM-1 gene in 14/30 isolates. All the above isolates carried the blaSHV-5 determinant as well; interestingly, 8/14 VIM positive isolates were also CTX-M-1- like producers. VIM-1 positive strains were present in all hospitals. PFGE genomic profiles of the 14/30 isolates showed that 2 different clones, A and B, were responsible for outbreaks. The coexistence in the same bacterial cell of compatible plasmids carrying epidemiologically important emerging resistance genes, such as MBLs and CTX-Ms, is worrisome since it could predict the generation and spread of pan-resistant bacteria and the consequent treatment option limitations that can lead to significant morbidity and mortality. Control measures should be applied to detect MBL-producing strains and to contrast the vertical and plasmidic diffusion of carbapenem-resistant K. pneumoniae in acute care and rehabilitation facilities.

Original languageEnglish
Pages (from-to)279-282
Number of pages4
JournalNew Microbiologica
Volume36
Issue number3
Publication statusPublished - Jul 2013

Keywords

  • ESβL
  • K. pneumonia
  • Metallo-β-lactamase

ASJC Scopus subject areas

  • Microbiology (medical)

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    Nucleo, E., Spalla, M., Piazza, A., Caltagirone, M. S., Asticcioli, S., Debiaggi, M., Matti, C., Daturi, R., Navarra, A., Labonia, M., & Migliavacca, R. (2013). Emergence of a VIM-1 MBL and CTX-M-15 ESβL-producing Klebsiella pneumoniae clone from acute and rehabilitation hospitals in Italy. New Microbiologica, 36(3), 279-282.