Emerging technologies for cancer research: Towards personalized medicine with microfluidic platforms and 3D tumor models

Matteo Turetta, Fabio Del Ben, Giulia Brisotto, Eva Biscontin, Michela Bulfoni, Daniela Cesselli, Alfonso Colombatti, Giacinto Scoles, Giuseppe Gigli, Loretta L. Del Mercato

Research output: Contribution to journalReview article

Abstract

In the present review, we describe three hot topics in cancer research such as circulating tumor cells, exosomes, and 3D environment models. The first section is dedicated to microfluidic platforms for detecting circulating tumor cells, including both affinity-based methods that take advantage of antibodies and aptamers, and “label-free” approaches, exploiting cancer cells physical features and, more recently, abnormal cancer metabolism. In the second section, we briefly describe the biology of exosomes and their role in cancer, as well as conventional techniques for their isolation and innovative microfluidic platforms. In the third section, the importance of tumor microenvironment is highlighted, along with techniques for modeling it in vitro. Finally, we discuss limitations of two-dimensional monolayer methods and describe advantages and disadvantages of different three-dimensional tumor systems for cell-cell interaction analysis and their potential applications in cancer management.

Original languageEnglish
JournalCurrent Medicinal Chemistry
Volume25
Issue number35
DOIs
Publication statusPublished - Jan 1 2018

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Keywords

  • 3D cell cultures
  • Cancer
  • Circulating tumor cells
  • Exosomes
  • Tumor microenvironment

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery
  • Organic Chemistry

Cite this

Turetta, M., Del Ben, F., Brisotto, G., Biscontin, E., Bulfoni, M., Cesselli, D., Colombatti, A., Scoles, G., Gigli, G., & Del Mercato, L. L. (2018). Emerging technologies for cancer research: Towards personalized medicine with microfluidic platforms and 3D tumor models. Current Medicinal Chemistry, 25(35). https://doi.org/10.2174/0929867325666180605122633