Emilin, a component of elastic fibers preferentially located at the elastin-microfibrils interface

Giorgio M. Bressan, Daniela Daga-Gordini, Alfonso Colombatti, Ines Castellani, Valeria Marigo, Dino Volpin

Research output: Contribution to journalArticle

Abstract

The fine distribution of the extracellular matrix glycoprotein emilin (previously known as glycoprotein gp115) (Bressan, G. M., I. Castellani, A. Colombatti, and D. Volpin. 1983. J. Biol. Chem. 258: 13262-13267) has been studied at the ultrastructural level with specific antibodies. In newborn chick aorta the protein was exclusively found within elastic fibers. In both post- and pre-embedding immunolabeling emilin was mainly associated with regions where elastin and microfibrils are in close contact, such as the periphery of the fibers. This localization of emilin in aorta has been confirmed by quantitative evaluation of the distribution of gold particles within elastic fibers. In other tissues, besides being associated with typical elastic fibers, staining for emilin was found in structures lacking amorphous elastin, but where the presence of tropoelastin has been demonstrated by immunoelectron microscopy. This was particularly evident in the oxitalan fibers of the corneal stroma, in the Descemet's membrane, and in the ciliary zonule. Analysis of embryonic aorta revealed the presence of emilin at early stages of elastogenesis, before the appearance of amorphous elastin. Immunofluorescence studies have shown that emilin produced by chick embryo aorta cells in culture is strictly associated with elastin and that the process of elastin deposition is severely altered by the presence of antiemilin antibodies in the culture medium. The name of the protein was derived from its localization at sites where elastin and microfibrils are in proximity (emilin, elastin microfibril interface located protein).

Original languageEnglish
Pages (from-to)201-212
Number of pages12
JournalJournal of Cell Biology
Volume121
Issue number1
Publication statusPublished - Apr 1993

Fingerprint

Microfibrils
Elastic Tissue
Elastin
Aorta
Glycoproteins
Tropoelastin
Descemet Membrane
Corneal Stroma
Immunoelectron Microscopy
Antibodies
Chick Embryo
elastin microfibril interface located protein
Gold
Names
Fluorescent Antibody Technique
Extracellular Matrix
Culture Media
Proteins
Cell Culture Techniques
Staining and Labeling

ASJC Scopus subject areas

  • Cell Biology

Cite this

Bressan, G. M., Daga-Gordini, D., Colombatti, A., Castellani, I., Marigo, V., & Volpin, D. (1993). Emilin, a component of elastic fibers preferentially located at the elastin-microfibrils interface. Journal of Cell Biology, 121(1), 201-212.

Emilin, a component of elastic fibers preferentially located at the elastin-microfibrils interface. / Bressan, Giorgio M.; Daga-Gordini, Daniela; Colombatti, Alfonso; Castellani, Ines; Marigo, Valeria; Volpin, Dino.

In: Journal of Cell Biology, Vol. 121, No. 1, 04.1993, p. 201-212.

Research output: Contribution to journalArticle

Bressan, GM, Daga-Gordini, D, Colombatti, A, Castellani, I, Marigo, V & Volpin, D 1993, 'Emilin, a component of elastic fibers preferentially located at the elastin-microfibrils interface', Journal of Cell Biology, vol. 121, no. 1, pp. 201-212.
Bressan GM, Daga-Gordini D, Colombatti A, Castellani I, Marigo V, Volpin D. Emilin, a component of elastic fibers preferentially located at the elastin-microfibrils interface. Journal of Cell Biology. 1993 Apr;121(1):201-212.
Bressan, Giorgio M. ; Daga-Gordini, Daniela ; Colombatti, Alfonso ; Castellani, Ines ; Marigo, Valeria ; Volpin, Dino. / Emilin, a component of elastic fibers preferentially located at the elastin-microfibrils interface. In: Journal of Cell Biology. 1993 ; Vol. 121, No. 1. pp. 201-212.
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