Emotional, endocrine and brain anandamide response to social challenge in infant male rats

Eva M. Marco, Maria Luisa Scattoni, Cinzia Rapino, Chiara Ceci, Nicole Chaves, Simone Macrì, Mauro Maccarrone, Giovanni Laviola

Research output: Contribution to journalArticle

Abstract

Individual response to stress is orchestrated by hypothalamus-pituitary axis corticosteroids, although critically modulated by the central endocannabinoid (eCB) system. Whilst the role of the eCB system in stress response and emotional homeostasis in adult animals has been extensively studied, it has only been scarcely investigated in developing animals. Herein, we aimed to investigate the participation of eCB ligands in the stress responses of neonate rats. Twelve days-old Wistar male rats were exposed to a social challenge (repeated brief isolations from dam and littermates), which resulted in a significant increase in serum corticosterone levels. This stressful social challenge also decreased spontaneous rat pups' behaviours and augmented isolation-induced ultrasonic vocalizations. Notably, a specific decrease in anandamide content (not 2-AG) was observed within the hippocampus (not in the striatum). However, the enhancement of eCB signalling by URB597 administration (0.1. mg/kg) did not affect the adrenocortical and behavioural responses to this postnatal social challenge. The influence of gestational stress was also evaluated in the infant offspring of rats dams exposed to restraint stress (PRS, three episodes/day, on gestation days 14 till delivery); however, PRS did not modify neonate responses to this postnatal challenge. Present findings provide evidence for the participation of the eCB system in the acute response to a social challenge in infant male rats. However, the lack of evidences from the pharmacological study encourages the investigation of alternative and/or indirect mechanisms that may participate in the behavioural and endocrine response to stress in developing animals. Further experiments are still needed to clarify the interactions between the HPA axis and the eCB system in stress reactivity at early postnatal stages.

Original languageEnglish
Pages (from-to)2152-2162
Number of pages11
JournalPsychoneuroendocrinology
Volume38
Issue number10
DOIs
Publication statusPublished - Oct 2013

Fingerprint

Endocannabinoids
Brain
Corticosterone
Psychological Stress
Ultrasonics
Hypothalamus
anandamide
Wistar Rats
Hippocampus
Adrenal Cortex Hormones
Homeostasis
Newborn Infant
Pharmacology
Ligands
Pregnancy
Serum

Keywords

  • Anandamide
  • Corticosterone
  • Emotion
  • Endocannabinoid system
  • Hippocampus
  • Neonate
  • Stress
  • Ultrasound vocalizations

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Psychiatry and Mental health
  • Biological Psychiatry
  • Endocrine and Autonomic Systems

Cite this

Marco, E. M., Scattoni, M. L., Rapino, C., Ceci, C., Chaves, N., Macrì, S., ... Laviola, G. (2013). Emotional, endocrine and brain anandamide response to social challenge in infant male rats. Psychoneuroendocrinology, 38(10), 2152-2162. https://doi.org/10.1016/j.psyneuen.2013.04.004

Emotional, endocrine and brain anandamide response to social challenge in infant male rats. / Marco, Eva M.; Scattoni, Maria Luisa; Rapino, Cinzia; Ceci, Chiara; Chaves, Nicole; Macrì, Simone; Maccarrone, Mauro; Laviola, Giovanni.

In: Psychoneuroendocrinology, Vol. 38, No. 10, 10.2013, p. 2152-2162.

Research output: Contribution to journalArticle

Marco, EM, Scattoni, ML, Rapino, C, Ceci, C, Chaves, N, Macrì, S, Maccarrone, M & Laviola, G 2013, 'Emotional, endocrine and brain anandamide response to social challenge in infant male rats', Psychoneuroendocrinology, vol. 38, no. 10, pp. 2152-2162. https://doi.org/10.1016/j.psyneuen.2013.04.004
Marco, Eva M. ; Scattoni, Maria Luisa ; Rapino, Cinzia ; Ceci, Chiara ; Chaves, Nicole ; Macrì, Simone ; Maccarrone, Mauro ; Laviola, Giovanni. / Emotional, endocrine and brain anandamide response to social challenge in infant male rats. In: Psychoneuroendocrinology. 2013 ; Vol. 38, No. 10. pp. 2152-2162.
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