EMT markers in lung adenocarcinoma pleural effusion spheroid cells

Enrico Giarnieri, Claudia De Vitis, Alessia Noto, Giuseppe Roscilli, Gerardo Salerno, Salvatore Mariotta, Alberto Ricci, Pierdonato Bruno, Giuseppe Russo, Andrea Laurenzi, Maria Rosaria Giovagnoli, Gennaro Ciliberto, Rita Mancini

Research output: Contribution to journalArticle

Abstract

Epithelial-to-mesenchymal transition (EMT) is a process in which cells undergo a developmental switch from epithelial to mesenchymal phenotype. This process has been related to embryologic morphogenesis but also to cancer progression and metastasis. The aim of the current study was to investigate the expression of EMT-related markers in adherent and spheroid cell cultures derived from malignant pleural effusions (MPEs) of patients affected by lung adenocarcinoma. On the basis of efficient in vitro propagation, six cases of MPEs were selected and analyzed by immunocytochemistry staining for EMT markers and by RT-PCR for transcription factors known to orchestrate EMT. EMT markers immunostaining showed in spheroids a statistically significant correlation between the loss of E-cadherin immunoreactivity and overexpression of N-cadherin (P

Original languageEnglish
Pages (from-to)1720-1726
Number of pages7
JournalJournal of Cellular Physiology
Volume228
Issue number8
DOIs
Publication statusPublished - Aug 2013

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Epithelial-Mesenchymal Transition
Pleural Effusion
Cadherins
Malignant Pleural Effusion
Cell culture
Transcription Factors
Switches
Morphogenesis
Cell Culture Techniques
Immunohistochemistry
Adenocarcinoma of lung
Staining and Labeling
Neoplasm Metastasis
Phenotype
Polymerase Chain Reaction
Neoplasms

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Giarnieri, E., De Vitis, C., Noto, A., Roscilli, G., Salerno, G., Mariotta, S., ... Mancini, R. (2013). EMT markers in lung adenocarcinoma pleural effusion spheroid cells. Journal of Cellular Physiology, 228(8), 1720-1726. https://doi.org/10.1002/jcp.24300

EMT markers in lung adenocarcinoma pleural effusion spheroid cells. / Giarnieri, Enrico; De Vitis, Claudia; Noto, Alessia; Roscilli, Giuseppe; Salerno, Gerardo; Mariotta, Salvatore; Ricci, Alberto; Bruno, Pierdonato; Russo, Giuseppe; Laurenzi, Andrea; Giovagnoli, Maria Rosaria; Ciliberto, Gennaro; Mancini, Rita.

In: Journal of Cellular Physiology, Vol. 228, No. 8, 08.2013, p. 1720-1726.

Research output: Contribution to journalArticle

Giarnieri, E, De Vitis, C, Noto, A, Roscilli, G, Salerno, G, Mariotta, S, Ricci, A, Bruno, P, Russo, G, Laurenzi, A, Giovagnoli, MR, Ciliberto, G & Mancini, R 2013, 'EMT markers in lung adenocarcinoma pleural effusion spheroid cells', Journal of Cellular Physiology, vol. 228, no. 8, pp. 1720-1726. https://doi.org/10.1002/jcp.24300
Giarnieri E, De Vitis C, Noto A, Roscilli G, Salerno G, Mariotta S et al. EMT markers in lung adenocarcinoma pleural effusion spheroid cells. Journal of Cellular Physiology. 2013 Aug;228(8):1720-1726. https://doi.org/10.1002/jcp.24300
Giarnieri, Enrico ; De Vitis, Claudia ; Noto, Alessia ; Roscilli, Giuseppe ; Salerno, Gerardo ; Mariotta, Salvatore ; Ricci, Alberto ; Bruno, Pierdonato ; Russo, Giuseppe ; Laurenzi, Andrea ; Giovagnoli, Maria Rosaria ; Ciliberto, Gennaro ; Mancini, Rita. / EMT markers in lung adenocarcinoma pleural effusion spheroid cells. In: Journal of Cellular Physiology. 2013 ; Vol. 228, No. 8. pp. 1720-1726.
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