TY - JOUR
T1 - Emx2 promotes symmetric cell divisions and a multipotential fate in precursors from the cerebral cortex
AU - Heins, Nico
AU - Cremisi, Federico
AU - Malatesta, Paolo
AU - Gangemi, Rosaria M R
AU - Corte, Giorgio
AU - Price, Jack
AU - Goudreau, Guy
AU - Gruss, Peter
AU - Götz, Magdalena
PY - 2001
Y1 - 2001
N2 - Distinct sets of precursor cells generate the mammalian cerebral cortex. During neurogenesis most precursors are specified to generate a single cell type and only few are multipotent. The cell-intrinsic molecular determinants of these distinct lineages are not known. Here we describe that retroviral transduction of the transcription factor Emx2 in precursors from the cerebral cortex results in a significant increase of large clones that are generated mostly by symmetric cell divisions and contain multiple cell types, comprising neurons and glial cells. Thus, Emx2 is the first cell-intrinsic determinant able to instruct CNS precursors towards a multipotential fate. To evaluate the role of endogenous Emx2 in cortical precursors, we examined cell division in Emx2-/- mice. These analyses further supported the role of endogenous Emx2 in the regulation of symmetric cell divisions in the developing cortex.
AB - Distinct sets of precursor cells generate the mammalian cerebral cortex. During neurogenesis most precursors are specified to generate a single cell type and only few are multipotent. The cell-intrinsic molecular determinants of these distinct lineages are not known. Here we describe that retroviral transduction of the transcription factor Emx2 in precursors from the cerebral cortex results in a significant increase of large clones that are generated mostly by symmetric cell divisions and contain multiple cell types, comprising neurons and glial cells. Thus, Emx2 is the first cell-intrinsic determinant able to instruct CNS precursors towards a multipotential fate. To evaluate the role of endogenous Emx2 in cortical precursors, we examined cell division in Emx2-/- mice. These analyses further supported the role of endogenous Emx2 in the regulation of symmetric cell divisions in the developing cortex.
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U2 - 10.1006/mcne.2001.1046
DO - 10.1006/mcne.2001.1046
M3 - Article
C2 - 11922140
AN - SCOPUS:0035655676
VL - 18
SP - 485
EP - 502
JO - Molecular and Cellular Neurosciences
JF - Molecular and Cellular Neurosciences
SN - 1044-7431
IS - 5
ER -