To study the relationship between graft vascular thrombosis and rejection, 33 female outbred pigs weighing 20-25 kg underwent total pancreatectomy and heterotopic spleno-duodeno-pancreatic transplantation (SDPTx) according to Dafoe's technique. Twenty of the 33 animals survived over one week and are being considered in this study: 10 animals (controls, group I) did not receive any immunosuppression, 10 (treated, group II) received cyclosporine (6 mg/kg i.v.) and azathioprine (25 mg/day via jejunostomy). Postoperatively fasting blood sugar, serum amylase, plasma electrolytes, Ht and intravenous glucose tolerance test (IVGTT) were determined. The animals of the two groups were sacrified between day 8 and day 28 after SDPTx in order to evaluate gross and microscopic allograft alterations. In group I severe rejection was a prominent finding in 9 out of 10 animals and it was associated with graft venous and arterial thrombosis. Two grafts had isolated venous thrombosis, 2 had only arterial thrombosis and 2 had combined venous and arterial thrombosis. In 8 grafts microscopic evidence of diffuse necrosis was present. The grafts of group II animals showed only minimal signs of rejection and no vascular thrombosis or necrosis. The histologic signs of rejection were always more severe in the duodenum than in the pancreas in both groups of animals. In group II the fasting blood levels at day 7 and IVCTT at day 14 after SDPTx were statistically lower then in group I (p <0,05). Vascular thrombosis seems to be correlated to rejection and a better control of the rejection should be the main objective to improve the survival in pancreas transplantation.
|Translated title of the contribution||En bloc allotransplantation of spleen, duodenum and pancreas in the pig|
|Number of pages||6|
|Publication status||Published - 1992|
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