Enantioseparation of 2-aryloxypropionic acids on chiral porous monolithic columns by capillary electrochromatography. Evaluation of column performance and enantioselectivity

Antonella Messina, Miroslav Flieger, Fiorella Bachechi, Massimo Sinibaldi

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The enantioseparation of 2-aryloxypropionic acids by capillary electrochromatography was tested on columns with a monolithic stationary phase prepared from silanized fused-silica capillaries (100 μm I.D.) by in situ copolymerization of glycidyl methacrylate, ethylene glycol dimethacrylate and methyl methacrylate in the presence of formamide and 1-propanol as the porogen solvents. The porous chiral monolithic stationary phases were prepared by reaction of the epoxy-groups at the surface of the monolith with (+)-1-(4-aminobutyl)-(5R,8S,10R)-terguride. To attain the minimum HETP values for the enantiodiscrimination of 2-phenoxypropionic acid, the influence of the composition of polymerization solution on column total porosity and efficiency was investigated. Optimum mobile phase conditions were found for all analytes tested using acetonitrile-methanol mixtures containing triethylamine and acetic acid as the buffer components. Furthermore, the chemical and mechanical stabilities of the columns were satisfactory, allowing hundreds of analyses.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalJournal of Chromatography A
Volume1120
Issue number1-2
DOIs
Publication statusPublished - Jul 7 2006

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Capillary Electrochromatography
1-Propanol
Methacrylates
Mechanical stability
Enantioselectivity
Porosity
Chemical stability
Fused silica
Polymerization
Silicon Dioxide
Acetic Acid
Copolymerization
Methanol
Buffers
Acids
Chemical analysis
ethylene dimethacrylate
glycidyl methacrylate
2-phenoxypropionic acid
dironyl

Keywords

  • 2-Aryloxypropionic acids
  • CEC enantioseparations
  • Chiral monolithic columns
  • Polymethacrylates

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Enantioseparation of 2-aryloxypropionic acids on chiral porous monolithic columns by capillary electrochromatography. Evaluation of column performance and enantioselectivity. / Messina, Antonella; Flieger, Miroslav; Bachechi, Fiorella; Sinibaldi, Massimo.

In: Journal of Chromatography A, Vol. 1120, No. 1-2, 07.07.2006, p. 69-74.

Research output: Contribution to journalArticle

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