Encapsulated living cells on microstructured surfaces

Silke Krol, Marc Nolte, Alberto Diaspro, Davide Mazza, Raffaella Magrassi, Alessandra Gliozzi, Andreas Fery

Research output: Contribution to journalArticlepeer-review

Abstract

The immobilization of cells in defined arrays (cell patterning) is a key step towards cell-based biosensors or other cell-based devices. While cell patterning is usually achieved by modifying the surface on which only the cells should adhere and leaving the cells unmodified, we present here a different approach in which cells are first coated with polyelectrolytes and subsequently immobilized on patterned surfaces. By coating, the cells are protected and their interactions with the substrate are modified such that patterning is simplified. We used microcontact printing of polyelectrolytes to structure surfaces such that regions of opposite charges and the same charge as the cell coating were present and found that we can thus achieve patterning of the coated yeast cells. In accordance with prior work, we find that coating does not kill the cells and coated GFP-expressing cells still function after immobilization, which we checked by fluorescence microscopy.

Original languageEnglish
Pages (from-to)705-709
Number of pages5
JournalLangmuir
Volume21
Issue number2
DOIs
Publication statusPublished - Jan 18 2005

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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