Encapsulated yeast cells inside Paramecium primaurelia: A model system for protection capability of polyelectrolyte shells

S. Krol, O. Cavalleri, P. Ramoino, A. Gliozzi, A. Diaspro

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

One of the most promising applications of encapsulated living cells is their use as protected transplanted tissue into the human body. A suitable system for the protection of living cells is the use of nano- or microcapsules of polyelectrolytes. These shells can be deposited easily on top of the cells by means of a layer-by-layer technique. An interesting feature of the capsules is the possibility to control their properties on a nanometre level, tuning their wall texture via the preparation conditions. Here we introduce a model system to test the protection ability of polyelectrolyte capsules. Common bakery yeast cells were encapsulated. They were coated with a fluorescently labelled shell at conditions known to guarantee cell survival, and the cell interior was stained with DAPI. The protozoan Paramecium primaurelia was incubated with this double-stained living yeast and visualized by means of two-photon excitation fluorescence microscopy. Cross-sections of the dye-stained material as well as autofluorescence of the fixed protozoan allowed us to follow the digestion of the coated yeast with time. Our investigation reveals that capsules prepared under these deposition conditions are permeable to lysosomal enzymes, leading to degradation of the yeast inside the intact capsules. Our preliminary results indicate the suitability of the introduced model as a test system of this permeability.

Original languageEnglish
Pages (from-to)239-243
Number of pages5
JournalJournal of Microscopy
Volume212
Issue number3
DOIs
Publication statusPublished - Dec 2003

Fingerprint

paramecia
Paramecium
yeast
capsules
Polyelectrolytes
Yeast
Capsules
Yeasts
Cells
Bakeries
Nanocapsules
Fluorescence microscopy
human body
cells
Cytoprotection
enzymes
permeability
Photons
Human Body
Fluorescence Microscopy

Keywords

  • Encapsulated living cells
  • Layer-by-layer polyelectrolyte deposition
  • Nanostructuring of multilayers
  • Paramecium primaurelia
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Instrumentation

Cite this

Encapsulated yeast cells inside Paramecium primaurelia : A model system for protection capability of polyelectrolyte shells. / Krol, S.; Cavalleri, O.; Ramoino, P.; Gliozzi, A.; Diaspro, A.

In: Journal of Microscopy, Vol. 212, No. 3, 12.2003, p. 239-243.

Research output: Contribution to journalArticle

Krol, S. ; Cavalleri, O. ; Ramoino, P. ; Gliozzi, A. ; Diaspro, A. / Encapsulated yeast cells inside Paramecium primaurelia : A model system for protection capability of polyelectrolyte shells. In: Journal of Microscopy. 2003 ; Vol. 212, No. 3. pp. 239-243.
@article{f39c524baec3474c86840d71a9f65edf,
title = "Encapsulated yeast cells inside Paramecium primaurelia: A model system for protection capability of polyelectrolyte shells",
abstract = "One of the most promising applications of encapsulated living cells is their use as protected transplanted tissue into the human body. A suitable system for the protection of living cells is the use of nano- or microcapsules of polyelectrolytes. These shells can be deposited easily on top of the cells by means of a layer-by-layer technique. An interesting feature of the capsules is the possibility to control their properties on a nanometre level, tuning their wall texture via the preparation conditions. Here we introduce a model system to test the protection ability of polyelectrolyte capsules. Common bakery yeast cells were encapsulated. They were coated with a fluorescently labelled shell at conditions known to guarantee cell survival, and the cell interior was stained with DAPI. The protozoan Paramecium primaurelia was incubated with this double-stained living yeast and visualized by means of two-photon excitation fluorescence microscopy. Cross-sections of the dye-stained material as well as autofluorescence of the fixed protozoan allowed us to follow the digestion of the coated yeast with time. Our investigation reveals that capsules prepared under these deposition conditions are permeable to lysosomal enzymes, leading to degradation of the yeast inside the intact capsules. Our preliminary results indicate the suitability of the introduced model as a test system of this permeability.",
keywords = "Encapsulated living cells, Layer-by-layer polyelectrolyte deposition, Nanostructuring of multilayers, Paramecium primaurelia, Saccharomyces cerevisiae",
author = "S. Krol and O. Cavalleri and P. Ramoino and A. Gliozzi and A. Diaspro",
year = "2003",
month = "12",
doi = "10.1111/j.1365-2818.2003.01251.x",
language = "English",
volume = "212",
pages = "239--243",
journal = "Journal of Microscopy",
issn = "0022-2720",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Encapsulated yeast cells inside Paramecium primaurelia

T2 - A model system for protection capability of polyelectrolyte shells

AU - Krol, S.

AU - Cavalleri, O.

AU - Ramoino, P.

AU - Gliozzi, A.

AU - Diaspro, A.

PY - 2003/12

Y1 - 2003/12

N2 - One of the most promising applications of encapsulated living cells is their use as protected transplanted tissue into the human body. A suitable system for the protection of living cells is the use of nano- or microcapsules of polyelectrolytes. These shells can be deposited easily on top of the cells by means of a layer-by-layer technique. An interesting feature of the capsules is the possibility to control their properties on a nanometre level, tuning their wall texture via the preparation conditions. Here we introduce a model system to test the protection ability of polyelectrolyte capsules. Common bakery yeast cells were encapsulated. They were coated with a fluorescently labelled shell at conditions known to guarantee cell survival, and the cell interior was stained with DAPI. The protozoan Paramecium primaurelia was incubated with this double-stained living yeast and visualized by means of two-photon excitation fluorescence microscopy. Cross-sections of the dye-stained material as well as autofluorescence of the fixed protozoan allowed us to follow the digestion of the coated yeast with time. Our investigation reveals that capsules prepared under these deposition conditions are permeable to lysosomal enzymes, leading to degradation of the yeast inside the intact capsules. Our preliminary results indicate the suitability of the introduced model as a test system of this permeability.

AB - One of the most promising applications of encapsulated living cells is their use as protected transplanted tissue into the human body. A suitable system for the protection of living cells is the use of nano- or microcapsules of polyelectrolytes. These shells can be deposited easily on top of the cells by means of a layer-by-layer technique. An interesting feature of the capsules is the possibility to control their properties on a nanometre level, tuning their wall texture via the preparation conditions. Here we introduce a model system to test the protection ability of polyelectrolyte capsules. Common bakery yeast cells were encapsulated. They were coated with a fluorescently labelled shell at conditions known to guarantee cell survival, and the cell interior was stained with DAPI. The protozoan Paramecium primaurelia was incubated with this double-stained living yeast and visualized by means of two-photon excitation fluorescence microscopy. Cross-sections of the dye-stained material as well as autofluorescence of the fixed protozoan allowed us to follow the digestion of the coated yeast with time. Our investigation reveals that capsules prepared under these deposition conditions are permeable to lysosomal enzymes, leading to degradation of the yeast inside the intact capsules. Our preliminary results indicate the suitability of the introduced model as a test system of this permeability.

KW - Encapsulated living cells

KW - Layer-by-layer polyelectrolyte deposition

KW - Nanostructuring of multilayers

KW - Paramecium primaurelia

KW - Saccharomyces cerevisiae

UR - http://www.scopus.com/inward/record.url?scp=0346970917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346970917&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2818.2003.01251.x

DO - 10.1111/j.1365-2818.2003.01251.x

M3 - Article

C2 - 14629549

AN - SCOPUS:0346970917

VL - 212

SP - 239

EP - 243

JO - Journal of Microscopy

JF - Journal of Microscopy

SN - 0022-2720

IS - 3

ER -