Encapsulation of mesenchymal stem cells from wharton's jelly in alginate microbeads

Letizia Penolazzi, Elisa Tavanti, Renata Vecchiatini, Elisabetta Lambertini, Fortunato Vesce, Roberto Gambari, Stefania Mazzitelli, Francesca Mancuso, Giovanni Luca, Claudio Nastruzzi, Roberta Piva

Research output: Contribution to journalArticle

Abstract

The description of a microencapsulation procedure for Wharton's jelly mesenchymal stem cells (WJMSCs) is reported. The applied method is based on the generation of monodisperse droplets by a vibrational nozzle. An ionic alginate encapsulation procedure was utilized for the microbeads hardening. Different experimental parameters were analyzed, including frequency and amplitude of vibration, polymer pumping rate, and distance between the nozzle and the gelling bath. The produced barium-alginate microbeads were characterized by excellent morphological characteristics as well as a very narrow size distribution. The microencapsulation procedure did not alter the morphology and viability of the encapsulated WJMSCs. In addition, the current paper reports the functional properties in terms of secretive profiles of both free and encapsulated WJMSCs. The analyzed factors were members of the family of interleukins, chemokines, growth factors, and soluble forms of adhesion molecules. These experiments showed that despite encapsulation, most of the proteins analyzed were secreted both by the free and encapsulated cells, even if in a different extent. In conclusion, the described encapsulation procedure represents a promising strategy to utilize WJMSCs for possible in vivo applications in tissue engineering and biomedicine.

Original languageEnglish
Pages (from-to)141-155
Number of pages15
JournalTissue Engineering - Part C: Methods
Volume16
Issue number1
DOIs
Publication statusPublished - Feb 1 2010

Fingerprint

Wharton Jelly
Alginate
Stem cells
Mesenchymal Stromal Cells
Microspheres
Encapsulation
Microencapsulation
Drug Compounding
Nozzles
Interleukins
Barium
Chemokines
Tissue engineering
Hardening
Tissue Engineering
Intercellular Signaling Peptides and Proteins
Polymers
Vibration
Baths
Adhesion

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Medicine(all)

Cite this

Penolazzi, L., Tavanti, E., Vecchiatini, R., Lambertini, E., Vesce, F., Gambari, R., ... Piva, R. (2010). Encapsulation of mesenchymal stem cells from wharton's jelly in alginate microbeads. Tissue Engineering - Part C: Methods, 16(1), 141-155. https://doi.org/10.1089/ten.tec.2008.0582

Encapsulation of mesenchymal stem cells from wharton's jelly in alginate microbeads. / Penolazzi, Letizia; Tavanti, Elisa; Vecchiatini, Renata; Lambertini, Elisabetta; Vesce, Fortunato; Gambari, Roberto; Mazzitelli, Stefania; Mancuso, Francesca; Luca, Giovanni; Nastruzzi, Claudio; Piva, Roberta.

In: Tissue Engineering - Part C: Methods, Vol. 16, No. 1, 01.02.2010, p. 141-155.

Research output: Contribution to journalArticle

Penolazzi, L, Tavanti, E, Vecchiatini, R, Lambertini, E, Vesce, F, Gambari, R, Mazzitelli, S, Mancuso, F, Luca, G, Nastruzzi, C & Piva, R 2010, 'Encapsulation of mesenchymal stem cells from wharton's jelly in alginate microbeads', Tissue Engineering - Part C: Methods, vol. 16, no. 1, pp. 141-155. https://doi.org/10.1089/ten.tec.2008.0582
Penolazzi L, Tavanti E, Vecchiatini R, Lambertini E, Vesce F, Gambari R et al. Encapsulation of mesenchymal stem cells from wharton's jelly in alginate microbeads. Tissue Engineering - Part C: Methods. 2010 Feb 1;16(1):141-155. https://doi.org/10.1089/ten.tec.2008.0582
Penolazzi, Letizia ; Tavanti, Elisa ; Vecchiatini, Renata ; Lambertini, Elisabetta ; Vesce, Fortunato ; Gambari, Roberto ; Mazzitelli, Stefania ; Mancuso, Francesca ; Luca, Giovanni ; Nastruzzi, Claudio ; Piva, Roberta. / Encapsulation of mesenchymal stem cells from wharton's jelly in alginate microbeads. In: Tissue Engineering - Part C: Methods. 2010 ; Vol. 16, No. 1. pp. 141-155.
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