Encapsulation of viable animal cells for hybrid bioartificial organs by the Biosil method

G. Carturan, G. Dellagiacoma, M. Rossi, R. Dal Monte, M. Muraca

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Gas phase silicon alkoxides react with the wet surface of mammalian cells, affording a stable and homogeneous layer of amorphous SiO2 modified by Si-CH3 and Si-H bonds. Layer thickness may be controlled by exposure time. The layer does not suppress cell viability or functionality, and may be applied to cells supported on a trapping network or to cell aggregates. H4-II-E-C3 rat hepatoma cells, Hep G2 human cancer cells and human fibroblasts on various supports were encapsulated by the SiO2 layer and studied in terms of glucose utilization and 3H- leucin incorporation into secreted proteins. In the case of pancreatic islets, encapsulation was carried out without supports, so that original islet volume and features were maintained. In vitro results indicate preservation of vitality and function, as tested by insulin production.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsB.S. Dunn, E.J.A. Pope, H.K. Schmidt, M. Yamane
Pages366-373
Number of pages8
Volume3136
DOIs
Publication statusPublished - 1997
EventSol-Gel Optics IV - San Diego, CA, United States
Duration: Jul 30 1997Aug 1 1997

Other

OtherSol-Gel Optics IV
CountryUnited States
CitySan Diego, CA
Period7/30/978/1/97

Fingerprint

Encapsulation
organs
animals
Animals
Cells
cells
Insulin
Fibroblasts
insulin
Glucose
Rats
alkoxides
fibroblasts
viability
glucose
rats
Proteins
Silicon
cancer
trapping

Keywords

  • Bioartificial organs
  • Biosil method
  • SiO encapsulation of cells

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Carturan, G., Dellagiacoma, G., Rossi, M., Dal Monte, R., & Muraca, M. (1997). Encapsulation of viable animal cells for hybrid bioartificial organs by the Biosil method. In B. S. Dunn, E. J. A. Pope, H. K. Schmidt, & M. Yamane (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3136, pp. 366-373) https://doi.org/10.1117/12.279168

Encapsulation of viable animal cells for hybrid bioartificial organs by the Biosil method. / Carturan, G.; Dellagiacoma, G.; Rossi, M.; Dal Monte, R.; Muraca, M.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / B.S. Dunn; E.J.A. Pope; H.K. Schmidt; M. Yamane. Vol. 3136 1997. p. 366-373.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Carturan, G, Dellagiacoma, G, Rossi, M, Dal Monte, R & Muraca, M 1997, Encapsulation of viable animal cells for hybrid bioartificial organs by the Biosil method. in BS Dunn, EJA Pope, HK Schmidt & M Yamane (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 3136, pp. 366-373, Sol-Gel Optics IV, San Diego, CA, United States, 7/30/97. https://doi.org/10.1117/12.279168
Carturan G, Dellagiacoma G, Rossi M, Dal Monte R, Muraca M. Encapsulation of viable animal cells for hybrid bioartificial organs by the Biosil method. In Dunn BS, Pope EJA, Schmidt HK, Yamane M, editors, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3136. 1997. p. 366-373 https://doi.org/10.1117/12.279168
Carturan, G. ; Dellagiacoma, G. ; Rossi, M. ; Dal Monte, R. ; Muraca, M. / Encapsulation of viable animal cells for hybrid bioartificial organs by the Biosil method. Proceedings of SPIE - The International Society for Optical Engineering. editor / B.S. Dunn ; E.J.A. Pope ; H.K. Schmidt ; M. Yamane. Vol. 3136 1997. pp. 366-373
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