Endocytosis of synaptic ADAM10 in neuronal plasticity and Alzheimer's disease

Elena Marcello, Claudia Saraceno, Stefano Musardo, Hugo Vara, Alerie Guzman De La Fuente, Silvia Pelucchi, Daniele Di Marino, Barbara Borroni, Anna Tramontano, Isabel Pérez-Otaño, Alessandro Padovani, Maurizio Giustetto, Fabrizio Gardoni, Monica Di Luca

Research output: Contribution to journalArticle

Abstract

A disintegrin and metalloproteinase 10 (ADAM10), a disintegrin and metalloproteinase that resides in the postsynaptic densities (PSDs) of excitatory synapses, has previously been shown to limit β-amyloid peptide (Aβ) formation in Alzheimer's disease (AD). ADAM10 also plays a critical role in regulating functional membrane proteins at the synapse. Using human hippocampal homogenates, we found that ADAM10 removal from the plasma membrane was mediated by clathrin-dependent endocytosis. Additionally, we identified the clathrin adaptor AP2 as an interacting partner of a previously uncharacterized atypical binding motif in the ADAM10 C-terminal domain. This domain was required for ADAM10 endocytosis and modulation of its plasma membrane levels. We found that the ADAM10/AP2 association was increased in the hippocampi of AD patients compared with healthy controls. Long-term potentiation (LTP) in hippocampal neuronal cultures induced ADAM10 endocytosis through AP2 association and decreased surface ADAM10 levels and activity. Conversely, long-term depression (LTD) promoted ADAM10 synaptic membrane insertion and stimulated its activity. ADAM10 interaction with the synapse-associated protein-97 (SAP97) was necessary for LTD-induced ADAM10 trafficking and required for LTD maintenance and LTD-induced changes in spine morphogenesis. These data identify and characterize a mechanism controlling ADAM10 localization and activity at excitatory synapses that is relevant to AD pathogenesis.

Original languageEnglish
Pages (from-to)2523-2538
Number of pages16
JournalJournal of Clinical Investigation
Volume123
Issue number6
DOIs
Publication statusPublished - Jun 3 2013

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Disintegrins
Neuronal Plasticity
Metalloproteases
Endocytosis
Alzheimer Disease
Synapses
Depression
Vesicular Transport Adaptor Proteins
Cell Membrane
Post-Synaptic Density
Synaptic Membranes
Clathrin
Long-Term Potentiation
Morphogenesis
Amyloid

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Marcello, E., Saraceno, C., Musardo, S., Vara, H., De La Fuente, A. G., Pelucchi, S., ... Di Luca, M. (2013). Endocytosis of synaptic ADAM10 in neuronal plasticity and Alzheimer's disease. Journal of Clinical Investigation, 123(6), 2523-2538. https://doi.org/10.1172/JCI65401

Endocytosis of synaptic ADAM10 in neuronal plasticity and Alzheimer's disease. / Marcello, Elena; Saraceno, Claudia; Musardo, Stefano; Vara, Hugo; De La Fuente, Alerie Guzman; Pelucchi, Silvia; Di Marino, Daniele; Borroni, Barbara; Tramontano, Anna; Pérez-Otaño, Isabel; Padovani, Alessandro; Giustetto, Maurizio; Gardoni, Fabrizio; Di Luca, Monica.

In: Journal of Clinical Investigation, Vol. 123, No. 6, 03.06.2013, p. 2523-2538.

Research output: Contribution to journalArticle

Marcello, E, Saraceno, C, Musardo, S, Vara, H, De La Fuente, AG, Pelucchi, S, Di Marino, D, Borroni, B, Tramontano, A, Pérez-Otaño, I, Padovani, A, Giustetto, M, Gardoni, F & Di Luca, M 2013, 'Endocytosis of synaptic ADAM10 in neuronal plasticity and Alzheimer's disease', Journal of Clinical Investigation, vol. 123, no. 6, pp. 2523-2538. https://doi.org/10.1172/JCI65401
Marcello, Elena ; Saraceno, Claudia ; Musardo, Stefano ; Vara, Hugo ; De La Fuente, Alerie Guzman ; Pelucchi, Silvia ; Di Marino, Daniele ; Borroni, Barbara ; Tramontano, Anna ; Pérez-Otaño, Isabel ; Padovani, Alessandro ; Giustetto, Maurizio ; Gardoni, Fabrizio ; Di Luca, Monica. / Endocytosis of synaptic ADAM10 in neuronal plasticity and Alzheimer's disease. In: Journal of Clinical Investigation. 2013 ; Vol. 123, No. 6. pp. 2523-2538.
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